Intracellular transport is critical to cellular functions and the maintenance of its integrity (membrane renewal, vesicles trafficking, cell division, mRNA transport, etc.). Among the molecular motors that are involved in intracellular transport, three large superfamilies have been identified and well characterized these last decades – kinesins, dyneins and myosins.
Kinesins, also known as KIFs, are microtubule-dependent molecular motors that use ATP as chemical fuel to transport cargo along the microtubule network. Indeed, five major kinesin families were initially described but there are as many as 45 mammalian kinesin genes to date. In most kinesins, the motor domain is found at the N-terminus (N-type). N-type kinesins are (+) end-directed motors, i.e. they transport cargo towards the (+) end of the microtubule. In the neuronal axon, synaptic vesicle precursors, mitochondria and protein complexes are transported bi-directionally. While retrograde transport is powered by dyneins, anterograde transport is essentially powered by kinesins.
Deciphering the regulation and functions of kinesins constitutes a major challenge and will broaden our understanding of molecular motors implications in intracellular transport.
Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, KNS, KNS1, Kinesin-1 heavy chain, UKHC, Anti-Kinesin-1 antibody, kinesin 1 antibody, kin1, kin-1, KIF5, KHC, Kinesin motor domain
Anti-Kinesin-1 was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human kinsesin-1 protein.