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Kinesin-1 Antibody

Rabbit Polyclonal
NCI Collaboration
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Western Blot of Rabbit anti-Kinesin 1 Antibody.  
Lane 1:  500 ng of truncated kinesin 1 protein.  
Lane 2:  20 ug of Mouse Brain lysate.  
Lane 3:  10 ug of Mouse Brain lysate.  
Primary antibody:  kinesin1 antibody at 1:1000 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:40,000 for 1hr at RT.
Block:  5% BLOTTO 1 hr at RT.
Predicted/Observed size:  72 kDa, ~70 kDa for kinesin-1. 
Other band(s): degradation.
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Number:
100-401-E80
Size:
100 µL
Price:
$319.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Kinesin-1 Antibody Properties

Description
Anti-Kinesin-1 (Rabbit) Antibody 100-401-E80
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:10000
Western Blot : 1:100-1:500
Immunohistochemistry: user optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
Antiserum
Label
Concentration
77 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
KIF5B
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Kinesin-1 Antibody Description

Background
Intracellular transport is critical to cellular functions and the maintenance of its integrity (membrane renewal, vesicles trafficking, cell division, mRNA transport, etc.). Among the molecular motors that are involved in intracellular transport, three large superfamilies have been identified and well characterized these last decades – kinesins, dyneins and myosins. Kinesins, also known as KIFs, are microtubule-dependent molecular motors that use ATP as chemical fuel to transport cargo along the microtubule network. Indeed, five major kinesin families were initially described but there are as many as 45 mammalian kinesin genes to date. In most kinesins, the motor domain is found at the N-terminus (N-type). N-type kinesins are (+) end-directed motors, i.e. they transport cargo towards the (+) end of the microtubule. In the neuronal axon, synaptic vesicle precursors, mitochondria and protein complexes are transported bi-directionally. While retrograde transport is powered by dyneins, anterograde transport is essentially powered by kinesins. Deciphering the regulation and functions of kinesins constitutes a major challenge and will broaden our understanding of molecular motors implications in intracellular transport.
Synonyms
Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, KNS, KNS1, Kinesin-1 heavy chain, UKHC, Anti-Kinesin-1 antibody, kinesin 1 antibody, kin1, kin-1, KIF5, KHC, Kinesin motor domain
Immunogen
Anti-Kinesin-1 was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human kinsesin-1 protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Kinesin-1 antibody has been tested for use in ELISA and western blot. For western blots expect a band of approximately 72 kDa in size corresponding to truncated kinesin-1 protein. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-Kinesin-1 is directed against the human kinesin-1 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with human. Cross-reactivity with kinesin 1 from other sources have not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Gindhart JG. (2006) Towards an Understanding of Kinesin-1 Dependent Transport Pathways Through the Study of Protein-Protein Interactions. Briefings in Fuctional Genomics and Proteomics. Vol 5. No 1. 74-86. Hirokawa N., Noda Y., Tanaka Y., and Niwa S. (2009) Kinesin Superfamily Motor Proteins and Intercellular Transport. Nature. Vol 10. 682-696. Clancy BE., Behke-Parks WM., Andreasson JOL., Rosenfeld SS., and Block SM. (2011) A universal pathway for kinesin stepping. 2011. Nature Structural Molecular Biology. Aug. 1-9.
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry