Conversion to Recombinant Antibody

Hybridoma or Purified Ab Sequencing

Rockland can convert your monoclonal antibody from a traditional hybridoma into a recombinant antibody format. This can be achieved by gene sequencing from an existing hybridoma cell line, or by de novo protein sequencing from purified antibody preparation. Once the antibody sequence is known, antibody genes in various formats can be synthesized and expressed to obtain recombinant antibody protein that is monoclonal and pure. Recombinant antibodies can also be generated from other discovery projects (i.e., immunization campaigns using various animal species), or selected using antibody display libraries generated from naïve (i.e., non-immunized), hyper-immunized animals, or synthetic antibody libraries.

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Targeting Genetic Drift: A Crucial Advantage

Rockland's long heritage and expertise in polyclonal & monoclonal antibody discovery and production now includes the conversion of your antibody into recombinant antibody (rAb) formats. By transitioning to a rAb format, the composition, integrity, production yield, quality, and performance reproducibility of your antibody can be improved and assured.

One of the most significant challenges in the use of traditional monoclonal antibodies is genetic drift—a random mutation process that occurs during continued cell divisions in hybridoma cell cultures. These mutations can modify the antibody’s binding sites, leading to decreased affinity and specificity over time. By transitioning to a recombinant platform, the genetic sequence of your antibody is cloned into a stable expression system, ensuring the preservation of its original properties indefinitely.

Choosing our recombinant antibody conversion service means investing in the stability and future of your research and products. Talk to our experts to learn more about how our recombinant conversion process can specifically protect your valuable antibodies from genetic drift.

Secure, High-Yield, and Customizable

Secure Supply & Immortalize Antibodies

Leverage advanced recombinant technology to provide a stable, continuous supply of high-quality antibodies.

Optimize Production

Enhance the yield from low-secreting hybridomas through efficient recombinant expression to scale up production.

Innovative Engineering

Customize antibodies to meet specific research and therapeutic objectives, with capabilities to modify class, species, or format.

De-Risk Genetic Drift

Recombinant technology stabilizes genetic material, preventing the drift that plagues traditional monoclonal antibody production.

Custom Recombinant Monoclonal Antibodies

rAbs can be produced in a wide range of formats that include common antibody constant regions (e.g., IgG1 Fc, IgG2 Fc, or others) as well as engineered molecular modifications such as fragments of the antibody (e.g., scFv) and inclusion of molecular tags (e.g., FLAG, poly-His, and a range of other options). Various other options include multi-valency and multi-specificity. We can also functionalize your rAbs via genetic fusions to other proteins or biochemically modify them with enzymes (e.g., horse radish peroxidase, alkaline phosphatase, and others), dyes (e.g., FITC and other fluorescent or chemiluminescent molecules) or other tags and molecules (e.g., biotin, PEG).

Whether your antibody is derived from a hybridoma cell line, a yeast or phage display library, or a de novo protein sequencing effort from a purified antibody preparation, we can improve the production and performance of your antibody by conversion to a rAb format.