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PTEN-P1 Antibody

Rabbit Polyclonal
NCI Collaboration
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Western blot using Rockland's affinity purified anti-PTEN-P1 antibody shows detection of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (lane 4), HEK293 (lane 5) and MCF7 (lane 6).  The band at ~55 kDa (arrowhead) corresponds to PTEN-P1.  Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide.  The identity of lower molecular weight bands in lane 4 is unknown.  Briefly, each lane contains approximately 35 µg of lysate.  Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.  The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature (800 nm channel, green).   Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).   IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.
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Number:
600-401-A90
Size:
100 µg
Price:
$319.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

PTEN-P1 Antibody Properties

Description
Anti-PTEN-P1 (RABBIT) Antibody - 600-401-A90
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:160,000
Western Blot : 1:500 to 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.15 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
PTENP1
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PTEN-P1 Antibody Description

Background
This gene (PTENP1) is a highly homologous pseudogene of PTEN.  PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. PTEN is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tension like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.
Synonyms
Phosphatase and tensin homolog pseudogene 1 antibody, psiPTEN antibody, PTEN rs antibody, PTEN2 antibody, PTH2 antibody, TEP1 antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminal end of human PTEN-P1 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to PTEN-P1 protein by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity-purified antibody is directed against human PTEN-P1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Reactivity occurs against human PTEN-P1 protein.   A BLAST analysis was used to suggest cross reactivity with PTEN proteins from mouse, dog, macaque, chimpanzee, rat, opossum, zebrafish, green pufferfish, and platypus sources based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Phillips,R.J., Mestas,J., Gharaee-Kermani,M., Burdick,M.D., Sica,A., Belperio,J.A., Keane,M.P. and Strieter,R.M. (2005) Epidermal growth factor and hypoxia-induced expression of CXC chemokine receptor 4 on non-small cell lung cancer cells is regulated by the phosphatidylinositol 3-kinase/PTEN/AKT/mammalian target of rapamycin signaling pathway and activation of hypoxia inducible factor-1alpha.  J. Biol. Chem. 280 (23), 22473-22481. Baak,J.P., Van Diermen,B., Steinbakk,A., Janssen,E., Skaland,I., Mutter,G.L., Fiane,B. and Lovslett,K. (2005) Lack of PTEN expression in endometrial intraepithelial neoplasia is correlated with cancer progression.  Hum. Pathol. 36 (5), 555-561. Steck,P.A., Pershouse,M.A., Jasser,S.A., Yung,W.K., Lin,H., Ligon,A.H., Langford,L.A., Baumgard,M.L., Hattier,T., Davis,T., Frye,C., Hu,R., Swedlund,B., Teng,D.H. and Tavtigian,S.V. (1997) Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers.  Nat. Genet. 15 (4), 356-362.
Specific Reference
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Product Type
Proteins and Peptides;
Applications
WB
Catalog Number
000-000-260

Product Type
Proteins and Peptides;
Catalog Number
000-000-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry