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HDAC-5 (internal) Antibody

Rabbit Polyclonal
NCI Collaboration
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Western Blot of Rabbit anti-HDAC5 antibody.  
Lane 1:  mouse brain extract.   
Load:  5 µg per lane.
Primary antibody:  HDAC5 antibody at 0.2µg/mL for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  124 kDa for HDAC5.
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Number:
600-401-J21
Size:
100 µg
Price:
$319.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

HDAC-5 (internal) Antibody Properties

Description
Anti-HDAC-5 (RABBIT) Antibody - 600-401-J21
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:60,000
IF Microscopy : 1:100-1:500
Western Blot : 0.2 µg/ml
Immunohistochemistry: 1:100-1:500
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.12 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer
50% (v/v) Glycerol
GeneName
HDAC5
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HDAC-5 (internal) Antibody Description

Background
HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions. Anti-HDAC5 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research.
Synonyms
Antigen NY-CO-9, EC 3.5.1.98, FLJ90614, HD5, histone deacetylase 5, KIAA0600, NY-CO-9
Immunogen
HDAC5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding phosphoserine 661 of human HDAC5.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-HDAC5 antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~124 kDa corresponding to the appropriate cell lysate or extract.
Purity/Specifity
Anti-HDAC5 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HDAC5. A BLAST analysis was used to suggest cross-reactivity with Human based on 100% sequence homology. Cross-reactivity with HDAC5 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Bertos NR, Wang AH, Yang XJ. (2001) Class II histone deacetylases: structure, function, and regulation. Biochem Cell Biol;79(3):243-52. [PMID: 11467738]. Grozinger CM, Schreiber SL. (2000) Regulation of histone deacetylase 4 and 5 and transcriptional activity by 14-3-3-dependent cellular localization . Proc Natl Acad Sci U S A;97(14):7835-40. Jul 5. [PMID: 10869435].
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human
Catalog Number
600-401-879

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-1000

Product Type
Molecular Biology Reagents;
Catalog Number
MB-068-0020

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry