Glutamine Synthetase Polyclonal Antibody-Western blot

Datasheet

Glutamine Synthetase Antibody

Name: Glutamine Synthetase Antibody
Brand: Rockland Immunochemicals
Availability: InStock

Goat Polyclonal

Item No. 200-101-238

Size: 50 mg

Applications: WB

Reactivity: Brevibacterium

Host Species: Goat

Please contact us for pricing

50 mg $0.00

25 µL $125.00

100 µg $475.00

Add to List

Bulk Orders

Availability: Ships next business day

Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Glutamine Synthetase antibody detects microbial GS. Glutamine synthetase (GS)is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine:Glutamate + ATP + NH3 → Glutamine + ADP + phosphate. Glutamine Synthetase uses ammonia produced by nitrate reduction, amino acid degradation, and photorespiration.[4] The amide group of glutamate is a nitrogen source for the synthesis of glutamine pathway metabolites. Anti-Glutamine Synthetase antibody is ideal for investigators involved in glucose energy metabolism research.

Expand Background

Specifications for Glutamine Synthetase Antibody

Product Details

Description: Anti-Glutamine Synthetase (GOAT) Antibody (BULK ORDER) - 200-101-238

Synonyms: goat anti-Glutamine Synthetase Antibody, Glutamine synthetase, GS

Host Species: Goat

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: glnA - View All glnA Products

Reactivity: Brevibacterium

Immunogen Type: Native Protein

Immunogen: Glutamine Synthetase [native from brevibacterium species]

Purity/Specificity: Anti-Glutamine Synthetase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.

Database Links:

NCBI - CAG33988.1

UniProtKB - Q79VE3

GeneID - 3345165

Application Details

Tested Applications: WB

Application Note: Anti-Glutamine Synthetase antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Glutamine Synthetase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product. It is suitable for Immunohistochemistry also. Specific conditions for reactivity should be optimized by the end user.

Formulation

Physical State: Lyophilized

Concentration: 10.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Reconstitution Volume: 5.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.