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Datasheet

CHK1 Antibody

Rabbit Polyclonal

600-401-906
100 µg
WB, ELISA
S. cerevisiae
Rabbit
$475.00 /Per Item
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Background

CHK1 (also known as serine/threonine-protein kinase CHK1 and checkpoint kinase 1) is involved in cell cycle arrest when DNA damage has occurred or when unligated DNA is present.  The kinase controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit. CHK1 is localized within the nucleus.

Product Details

Anti-CHK1 Yeast (RABBIT) Antibody - 600-401-906
rabbit anti-CHK1 Antibody, CHK-1, CHK 1, Serine/threonine-protein kinase CHK1, Checkpoint kinase 1
Rabbit
Polyclonal
IgG

Target Details

CHK1  - View All CHK1 Products
S. cerevisiae
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 305-330 of S. cerevisiae CHK1.
This affinity purified antibody is directed against yeast CHK1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against yeast CHK1 protein.  A BLAST analysis was used to suggest little to no cross reactivity with this protein from other sources.

Application Details

ELISA, WB
This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 40-60 kDa in size corresponding to CHK1 by western blotting in the appropriate cell lysate or extract.

Formulation

Liquid (sterile filtered)
0.73 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.