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cdk2 Antibody

Rabbit Polyclonal

100-401-161S 100-401-161
25 µL 100 µL
WB, IHC
Human, Mouse, Rat
Rabbit
$125.00 /Per Item
$475.00 /Per Item
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Background

Anti cdk2 antibody recognizes a protein encoded by a gene member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

Product Details

Anti-CDK2 (RABBIT) Antibody - 100-401-161
rabbit anti-Cdk2 Antibody, Cdc2 related protein kinase antibody, Cdk 2 antibody, CDKN2 antibody, Cell division kinase 2 antibody, Cell division protein kinase 2 antibody, rabbit anti-Cyclin dependent kinase 2 antibody, p33 protein kinase antibody, cdk
Rabbit
Polyclonal
Antiserum

Target Details

CDK2  - View All CDK2 Products
Human, Mouse, Rat
Conjugated Peptide
Anti-cdk2 antibody was prepared from whole rabbit serum by repeated immunizations with a synthetic peptide corresponding to the C-terminus of the human cdk-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Anti-cdk2 antibody was prepared from monospecific antiserum by delipidation and defibrination.  Antiserum will specifically react with a 33 kDa cdk2 protein from human, rat and mouse tissue.  No reaction was observed against other related cyclin dependent kinases.  Cross reactivity with cdk2 from other species may also occur.

Application Details

IHC, WB
Anti-cdk2 antibody has been tested in western blot and immunohistochemistry and is suitable for IF and other antibody based assays.  Multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Use paraffin embedded tissues for immunohistochemistry.  Optimal titers for other applications should be determined by the researcher.

Formulation

Liquid (sterile filtered)
75 mg/mL by Refractometry
None
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.