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Supporting Reagents


 

Supporting Reagents

 

Rockland offers supporting reagents to facilitate various immunoassays including Blocking Solutions, BSA and Enzyme Substrates.  Carefully formulated by our scientists, the use of these essential assay components dramatically increases the quality and reproducibility of your experiments.  Blocking solutions are essential for use in ELISA, western blotting, immunohistochemistry and many other antibody dependent assays.  Enzyme substrates allow for soluble or insoluble color development to localize and quantitate signal detection when horseradish peroxidase, alkaline phosphatase or beta galactosidase are used in direct or indirect immunoassays.  Enzyme substrates are available that result in either soluble or insoluble color formation depending on the requirements of the assay.

 

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Featured Supporting Reagents

 

 

 

 


 

Blocking Solutions and BSA

 

Blocking solutions are used to reduce nonspecific binding.  Because there is no universal blocking reagent, Rockland offers a selection of blocking reagents so that the investigators can choose the most appropriate reagent for a specific assay.  Optimizing a blocking buffer requires monitoring of both background (negative control) and signal strength (positive control) to compare blockers.  Choose the blocker that produces the highest signal to noise ratio.

 

 

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   Blocking Solutions and BSA

 

  

Applications

 

Blocking Grade Normal Serum as Blocking Reagents and Diluents
Bovine Serum Albumin (BSA) as Blocking Reagents and Diluents
BLOTTO Immunoanalytical grade NFDM as Blocking Reagents
Blocking Buffer for Western Blotting
ELISA Microwell Blocking Buffers and Stabilizers



Enzyme Substrates

 

Enzyme substrates are used in colorimetric, fluorometric or luminescent assays and are reagents that undergo a measurable color change or emit light in in the presence of an antibody bound to analytes.  Enzyme substrates that produce insoluble products are used to localize targets in assays like immunoblotting and immunohistochemistry.  Enzyme substrates that produce soluble products are used to quantitate targets in assays like ELISA or FLISA.  Substrates that are acted upon by beta galactosidase are often used in molecular biology, microbiology or immunology.

 

 

Enzyme Substrates, view all

 

   Enzyme Substrates

 

Applications

 

Colorimetric Enzyme Substrates for Horseradish Peroxidase
Colorimetric Enzyme Substrates for Alkaline Phosphatase
Colorimetric Enzyme Substrates for Beta galactosidase
Chemiluminescent Enzyme Substrates for Horseradish Peroxidase


 

Protein A and Protein G Reagents

 

Protein A and protein G are proteins that bind IgG, IgM and other immunoglobins.  Both Protein A and Protein G exhibit different binding properties for antibodies from various species. Rockland offers protein A and protein G in unconjugated and conjugated forms for many common immunoassays.  Both Protein A and Protein G are also available conjugated to Sepharose beads and can be used to purify antibodies directly from serum or cell culture supernatant fluid.

 

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   Protein A Peroxidase

 

Applications

 

Protein A conjugated to HRP for western blotting

Protein G conjugated to Fluorescein for immunofluorescence microscopy  

Protein A conjugated to Sepharose for purification

Protein G conjugated to Biotin for ELISA

 

 

 

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