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Secondary Antibodies  >  Fluorescein Conjugated Antibodies
 

Rabbit IgG (H&L) Secondary Antibody Fluorescein Conjugated

Goat Polyclonal
Code

611-1202
Size

2 mg
Price

$99.00
Availability

Ships next business day.
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Rockland FITC (fluorescein) and HRP (horse radish peroxidase) conjugated secondary antibody was used to detect nanogram – picogram levels of rabbit IgG by dot blot on nitrocellulose membrane.  4 ul each of serial 1 in 4 dilutions of rabbit IgG were dotted on nitrocellulose and allowed to dry.  Membrane was blcoked in 3% BSA for 10 minutes dried for later use and rewetted with MB-070.  Blot was incubated in Rockland fluorescein conjugated goat anti rabbit 611-1202 lot 25176 1:10,000 and Rockland HRP conjugated goat anti Rabbit (611-1302 lot 25406 1:10,000, dried and:

A.  Blot was imaged on the BioRad VersaDoc with filter settings appropriate for Fluorescein/DyLight 488
B.  Blot was rewetted with TBS, incubated with FEMTOMAX chemiluminescent substrate for 1-3 minutes and imaged for 60sec on the BioRad VersaDoc Imaging System
C.   Blot was rinsed with TBS and DIH2O, incubated for 5 minutes with Rockland TMB Substrate for Western Blot MaxTag (1 ml of TMBM-102 + ~9 ml of TMBM-101), dried overnight and imaged using a conventional flatbed scanner
Western blot of Fluorescein conjugated Goat Anti-Rabbit IgG secondary antibody.
Lane 1:  Rabbit IgG.
Lane 2:  None.
Load:  50 ng per lane.
Primary antibody:  None.
Secondary antibody:  Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT.
Blocking:  MB-070 for 30 min at RT.
Predicted/Observed size:  25 & 55 kDa, 25 & 55 kDa for Rabbit IgG.
Other band(s):  None.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: FITC, fluorescein

Rabbit IgG (H&L) Secondary Antibody Fluorescein Conjugated Properties

Description
Anti-RABBIT IgG (H&L) (GOAT) Antibody Fluorescein Conjugated - 611-1202
Host
Goat
Specificity
IgG (H&L)
Target Species
Rabbit
Known Cross Reactivity
Rabbit
Clonality
Polyclonal
Application
FLISA : 1:10,000 - 1:50,000
IF Microscopy : 1:1,000 - 1:5,000
Flow Cytometry : 1:500 - 1:2,500
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
FP Value
3.7 moles Fluorescein (FITC) per mole of IgG
Concentration
2.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

Rabbit IgG (H&L) Secondary Antibody Fluorescein Conjugated Description

Background
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Immunogen
Rabbit IgG whole molecule
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Fluorescein (FITC)
Color
Green
Conjugation Reference
Modified from The and Feltkamp, Immunology 18; 865, 1970.
Molecular Weight
390
Excitation Wavelength
495
Conjugation Chemistry
Isothiocyanate