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Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed

Goat Polyclonal
Code

611-103-122
Size

1 mg
Price

$205.00
Availability

Ships next business day.
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Rockland secondary antibodies detect rabbit primary antibodies in a variety of platforms.  Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with Rockland HRP conjugated Goat anti Rabbit IgG  (611-103-122) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070.  Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649),
(B) LiCor Odyssey Reader (700 nm),
(C )  Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec),
(D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and 
(E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager,
HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer  to detect a rabbit primary antibody by Western Blot.  Anti p27 antibody (200-401-C30, 1:1000 RT 30 minutes) showed  detection of 0.1 µg of recombinant p27 protein.  Lane 1: Molecular weight markers.  Lane 2:  MBP-p27 fusion protein (arrow; expected MW: 73.3 kDa).  Lane 3:  MBP alone.  Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose and blocked with 1% BSA-TTBS (p/n  MB-013, diluted to 1X) overnight at 4°C.  Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
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Synonyms: goat anti rabbit hrp, HRP conjugated secondary antibody

RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed Properties

Description
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) - 611-103-122
Host
Goat
Specificity
IgG (H&L)
Target Species
Rabbit
Clonality
Polyclonal
Application
ELISA : 1:90,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed Description

Background
Anti Rabbit HRP Conjugated Secondary Antibodies are ideal for Western Blotting, Immunohistochemistry, ELISA as well as other anibody detection methods.
Immunogen
Rabbit IgG whole molecule
Storage Condition
Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Purity/Specifity
Conjugated Second Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate