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Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

MOUSE IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated

Rabbit Polyclonal
Code

610-4502
Size

1 mg
Price

$149.00
Availability

Ships next business day.
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Western Blot of Rabbit anti-Mouse IgG Antibody Alkaline Phosphatase Conjugated
Lane 1:  Mouse IgG 
Load:  100 ng per lane
Secondary antibody:  MOUSE IgG (H&L) Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT
Block:  MB-070 for 30 min at RT
Predicted/Observed size:  55 and 28 kDa, 55 and 28 kDa
Shipping & Handling Fees Apply.

Shipping:
$20.00 to United States
Handling:
$20.00
Synonyms: Conjugated Secondary Antibody, Alk Phos anti mouse IgG, ALP antibody conjugate

MOUSE IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Properties

Description
Anti-MOUSE IgG (H&L) (RABBIT) Antibody Alkaline Phosphatase Conjugated - 610-4502
Host
Rabbit
Specificity
IgG (H&L)
Target Species
Mouse
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:10,000
Western Blot : 1:500 - 1:2,500
Immunohistochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

MOUSE IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Description

Background
Conjugated Secondary Antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Immunogen
Mouse IgG whole molecule
Storage Condition
Store secondary antibody at 4° C before opening. DO NOT FREEZE. Mouse conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Application Note
Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Purity/Specifity
Secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Alkaline Phosphatase (Calf Intestine)
Color
Substrate dependent
Conjugation Reference
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175, 1971.
Molecular Weight
140000
Excitation Wavelength
Conjugation Chemistry
Glutaraldehye coupling