Secondary Antibodies  >  Fluorescein Conjugated Antibodies
 

CHICKEN IgG (H&L) Secondary Antibody Fluorescein Conjugated

Rabbit Polyclonal
NCI Collaboration JCYIA Product
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  • Western Blot of Anti-Chicken IgG (H&L) (RABBIT) Antibody
Number:
603-4202
Size:
1.5 mg
Price:
$126.00
Shipping:
$32.00
Handling:
$20.00
Availability:
Ships next business day.

CHICKEN IgG (H&L) Secondary Antibody Fluorescein Conjugated Properties

Description
Anti-CHICKEN IgG (H&L) (RABBIT) Antibody Fluorescein Conjugated - 603-4202
Host
Rabbit
Specificity
IgG (H&L)
Target Species
Chicken
Clonality
Polyclonal
Application
FLISA : 1:10,000 - 1:50,000
IF Microscopy : 1:1,000 - 1:5,000
Flow Cytometry : 1:500 - 1:2,500
Other Dilution: FLOW CYTOMETRY 1:500 - 1:2,500
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
FP Value
3.0 moles Fluorescein (FITC) per mole of IgG
Concentration
1.5 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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CHICKEN IgG (H&L) Secondary Antibody Fluorescein Conjugated Description

Background
Anti-Chicken IgG Fluorescein Antibody generated in rabbit detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Synonyms
rabbit anti-Chicken IgG Antibody Fluorescein Conjugation, rabbit anti-Chicken IgG FITC Conjugated Antibody
Immunogen
Chicken IgG whole molecule
Storage Condition
Store Secondary Antibody at 4° C prior to restoration.   For extended storage aliquot antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Purity/Specifity
Anti-Chicken IgG/IgY Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Chicken IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, FC
Catalog Number
BSA-50

Product Label

 

Description
Fluorescein (FITC)
Color
Green
Conjugation Reference
Modified from The and Feltkamp, Immunology 18; 865, 1970.
Molecular Weight
390
Excitation Wavelength
495
Conjugation Chemistry
Isothiocyanate