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UBE2J1 Antibody

Rabbit Polyclonal
NCI Collaboration
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Most modifiers mature by proteolytic processing from inactive precursors (“a” = amino acid).  Arrowheads point to the cleavage sites.  Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins.  Conjugation requires activating (E1) and conjugating (E2) enzymes that form thioesters (S) with the modifiers.  Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein)/CBC(cullin-2/elonginB/elonginC)-like E3 enzymes that are also involved in ubiquitination.  In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains.  UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail.  Whether HUB1 functions as a modifier is currently unclear.  APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.  (From Jentsch & Pyrowolakis (2000); see references below.)
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Number:
600-401-991S
Size:
25 µL
Price:
$95.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

UBE2J1 Antibody Properties

Description
Anti-Ubiquitin-Conjugating Enzyme E2 J1 (Ube2j1) [RABBIT) Antibody - 600-401-991S
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:1,000 - 1:5,000
Western Blot : 1:200 - 1:2,000
ImmunoPrecipitation: 1-2 µg
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
0.71 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
UBE2J1
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UBE2J1 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Ube2j1 and Ube2j2 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal transmembrane domain.  By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response.
Synonyms
Non-canonical ubiquitin-conjugating enzyme 1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the amino terminus of the human Ube2j1 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 35-40 kDa in size corresponding to Ube2j1 protein by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity purified antibody is directed against human Ube2j1 protein.  The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest cross-reactivity with Ube2j1 protein from human, mouse and rat based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Lenk,U., Yu,H., Walter,J., Gelman,M.S., Hartmann,E., Kopito,R.R. and Sommer,T. (2002) A role for mammalian Ubc6 homologues in ER-associated protein degradation. J. Cell Sci. 115:3007-3014. Lester,D., Farquharson,C., Russell,G. and Houston,B. (2000) Identification of a family of noncanonical ubiquitin-conjugating enzymes structurally related to yeast UBC6. Biochem. Biophys. Res. Commun. 269(2):474-480. Jentsch, S. and Pyrowolakis, G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-342.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry