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PPAR Gamma 1 + 2 Antibody

Rabbit Polyclonal
NCI Collaboration
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Number:
600-401-419
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

PPAR Gamma 1 + 2 Antibody Properties

Description
Anti-PPAR gamma 1 and 2 (internal) (RABBIT) Antibody - 600-401-419
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat, squirrel, orangutan, duck, macaque, boar, mink, guinea pig, rabbit, dog, hamster
Clonality
Polyclonal
Application
ELISA : 1:8,000 - 1:32,000
Western Blot : User optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
PPAR
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PPAR Gamma 1 + 2 Antibody Description

Background
Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones.  Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma.  Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.
Synonyms
HUMPPARG antibody, NR1C3 antibody, PPAR gamma antibody, PPARG antibody, PPARG1 antibody, PPARG2 antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 255 -268 of human PPAR gamma isoform 1.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity-purified antibody has been tested for use in ELISA.  Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
This affinity purified antibody is directed against human PPAR gamma protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest broad reactivity with this protein from several sources.  Expect reactivity against human (g1 and g2), mouse (g1 and g2), rat, squirrel, orangutan, duck (g1), macaque (g1-g7), boar (g1a-1d), mink, guinea pig, rabbit (g1 and g3), dog and hamster sources based on 100% homology for the immunogen sequence.  Cross reactivity with PPAR gamma homologues from other sources has not been determined.  No reactivity is expected against other subtypes of PPAR.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
000-000-264

Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-306

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-321

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-264

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry