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PPAR Gamma 2 Antibody

Rabbit Polyclonal
NCI Collaboration
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Western blot using Rockland's affinity purified anti-PPARg2 antibody shows detection of PPARg2 protein in a mouse 3T3 whole cell lysate (lane 1 arrowhead).  Approximately 20 µg of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose.  Primary antibody was used at a 1:2,000 dilution in 5% BLOTTO PBS solution. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10.  Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M).  Other detection systems will yield similar results.
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Number:
600-401-418
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

PPAR Gamma 2 Antibody Properties

Description
Anti-PPAR gamma 2 (N-terminal specific) (RABBIT) Antibody - 600-401-418
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, macaque
Clonality
Polyclonal
Application
ELISA : 1:4,000 - 1:40,000
Western Blot : 1:1,000 - 1:3,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
PPARG
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PPAR Gamma 2 Antibody Description

Background
Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones.  Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.
Synonyms
NR1C3 antibody, peroxisome proliferative activated receptor gamma isoform 2 antibody, Peroxisome proliferator activated receptor gamma 2 antibody, PPARG antibody, PPARG2 antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human PPAR gamma 2.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a single band approximately 55 kDa in size corresponding to PPARg2 by western blot in the appropriate tissue or cell lysate.
Purity/Specifity
This affinity purified antibody is directed against human PPAR gamma isoform 2 protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest reactivity with this protein from human and macaque based on 100% homology for the immunogen sequence. Cross reactivity with PPARg2 protein from dog and swine is likely due to 94% homology (15/16 identities) with the protein from these sources.   Cross reactivity does occur with PPARg2 from mouse.  Mouse PPARg2 shows 87% homology to the immunogen.  Cross reactivity with PPARg2 homologues from other sources has not been determined.  No reactivity is expected against other subtypes of PPAR.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
General Reference(s): De Pascale,M.C., Bassi,A.M., Patrone,V., Villacorta,L., Azzi,A. and Zingg,J.M. (2006) Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes. Arch. Biochem. Biophys. 447 (2), 97-106. Lodillinsky,C., Umerez,M.S., Jasnis,M.A., Casabe,A., Sandes,E. and Eijan,A.M. (2006) Bacillus Calmette-Guerin induces the expression of peroxisome proliferator-activated receptor gamma in bladder cancer cells. Int. J. Mol. Med. 17 (2), 269-273. Schild,R.L., Sonnenberg-Hirche,C.M., Schaiff,W.T., Bildirici,I., Nelson,D.M. and Sadovsky,Y. (2006) The kinase p38 regulates peroxisome proliferator activated receptor-gamma in human trophoblasts. Placenta 27 (2-3), 191-199. Pandhare,J., Cooper,S.K. and Phang,J.M. (2006) Proline oxidase, a proapoptotic gene, is induced by troglitazone: evidence for both peroxisome proliferator-activated receptor gamma-dependent and -independent mechanisms. J. Biol. Chem. 281 (4), 2044-2052. Sastre,M., Dewachter,I., Rossner,S., Bogdanovic,N., Rosen,E., Borghgraef,P., Evert,B.O., Dumitrescu-Ozimek,L., Thal,D.R., Landreth,G., Walter,J., Klockgether,T., van Leuven,F. and Heneka,M.T. (2006) Nonsteroidal anti-inflammatory drugs repress beta-secretase gene promoter activity by the activation of PPARgamma. Proc. Natl. Acad. Sci. U.S.A. 103 (2), 443-448.
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
000-000-264NP

Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-306

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Human
Catalog Number
100-301-176

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-264

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry