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Primary Antibodies  >  Transcription Factor Antibodies
 

TMBIM1 Antibody

Rabbit Polyclonal
Code

200-401-A57
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Immunohistochemistry of Rabbit anti-TMBIM1 antibody.  
Tissue:  prostate.  
Fixation:  formalin fixed paraffin embedded.  
Antigen retrieval:  not required.
Primary antibody:  anti-TMBIM1 antibody  at 10 µg/mL  for 1 h at RT.
Secondary antibody:  Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.
Staining:  TMBIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.
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Synonyms: Putative uncharacterized protein TMBIM1

TMBIM1 Antibody Properties

Description
Anti-TMBIM1 (RABBIT) Antibody - 200-401-A57
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:10,000
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 10µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.2 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
TMBIM1
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Lot#  

TMBIM1 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. TMBIM1 (Transµembrane BAX inhibitor µotif-containing protein 1) is a member of the 7 TMS (7 transmembrane domains) family of receptors known to mediate the activation of various transcription factors. TMBIM1 is identified as a novel modulator of NF-kB activation.  A drosophila homolog of this protein (dNMDA1 with 40% homology to hTMBIM1) is reported to be unregulated during aging and oxidative stress.
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of human TMBIM1 protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein A purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 35 kDa in size corresponding to TMBIM-1 by western blotting in the appropriate cell lysate or extract.  To date this antibody has shown the ability to recognize over-expressed TMBIM1 but not endogenous protein.
Purity/Specifity
This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads.  This antibody is specific for human TMBIM1 protein.  A BLAST analysis was used to suggest partial cross-reactivity with TMBIM1 from mouse and rat sources based on ~88% homology with the immunizing sequence.  Cross-reactivity with TMBIM1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
Human
Catalog Number
200-C01-B34

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Cell Lysates;
Applications
WB
Catalog Number
W09-000-364

Product Type
Cell Lysates;
Applications
WB
Catalog Number
W09-001-367

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry