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Proteins and Peptides  >  Cytokines and other Growth Factors
 

VEGF-121 Human Recombinant Protein

Other - E.coli
Code

009-001-C23
Size

10 µg
Price

$225.00
Availability

Ships within 2 weeks.
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SDS-PAGE shows band corresponding to VEGF (1µg) in lane 1 (unreduced, arrowhead) and lane 3 (reduced).   Molecular weight estimation was made by comparison to prestained MW markers, lane 2.
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Synonyms: Vascular endothelial growth factor A-121, VEGF-A121 cytokine, Vascular permeability factor, VPF-121

VEGF-121 Human Recombinant Protein Properties

Description
VEGF-121 Human Recombinant Protein - 009-001-C23
Host
Other - E.coli
Known Cross Reactivity
Human
Application
Other Dilution: User Optimized
Species of Origin
Human
Physical State
Lyophilized
Shipping condition
Ambient
PSC Type
Cytokine, Interleukin or Growth Factor
PSC Format
Recombinant Protein
Label
Concentration
0.1 mg/mL by UV absorbance at 280 nm
Buffer
None
Reconstitution Volume
200 µL to 40 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
VEGFA
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VEGF-121 Human Recombinant Protein Description

Background
Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth, vascular permeability, cell migration, and endothelial cell proliferation and growth, it may play a role in stimulating vasolidation via nitric oxide-dependent pathways and in inhibition of apoptosis. VEGF binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. VEGF121 is acidic, freely secreted, and widely expressed. This isoform is produced by alternative promoter usage and alternative initiation. It starts at an alternative upstream CUG codon and is post-translationally processed to produce the secreted VEGF peptide and a N-terminal peptide N-VEGF. The unprocessed protein and the N-VEGF peptide may localize to the nucleus, the endoplasmic reticulum and the Golgi or the extracellular matrix. Recombinant Human VEGF-121 produced in E.coli is a double, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 28423 Daltons.
Storage Condition
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Application Note
VEGF-121 is suitable as a control for polyclonal or monoclonal anti-VEGF-121 in immunological assays.
Purity/Specifity
Purity was determined to be greater than 95% as determined by analysis by RP-HPLC, and by reducing and non-reducing SDS-PAGE, against known standard.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Sato,J.D. and Whitney,R.G. (1999) Direct Submission Human cDNA for vascular endothelial growth factor isoform VEGF121. Unpublished Submitted: Division of Cell, Molecular and Developmental Biology, American Type Culture Collection, 10801 University Boulevard, Manassas, VA 20110, USA Rosenbaum-Dekel Y., Fuchs A., Yakirevich E., Azriel A., Mazareb S., Resnick M.B., Levi B.Z. (2005) Nuclear localization of long-VEGF is associated with hypoxia and tumor angiogenesis. Biochem. Biophys. Res. Commun. 332:271-278(2005) [PubMed: 15896327] [Abstract]
Specific Reference
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Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry