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Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

Goat Polyclonal
NCI Collaboration
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Dot Blot of Goat anti-Mouse IgG Antibody Phycoerythrin Conjugated.  
Antigen:  Mouse IgG.  
Load:  3-fold serial dilution starting at 200 ng.
Primary antibody:  None.
Secondary antibody:   Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT.
Block:  5% BLOTTO 1 Hour at RT.
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Number:
610-108-121
Size:
1 mL
Price:
$215.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed Properties

Description
Anti-MOUSE IgG [H&L] (GOAT) Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Hs Ham Hu Rb Rt &Sh Serum Proteins - 610-108-121
Host
Goat
Specificity
IgG (H&L)
Target Species
Mouse
Known Cross Reactivity
Mouse
Clonality
Polyclonal
Application
IF Microscopy : 1:100 - 1:250
Flow Cytometry : 1:100 - 1:250
Other Dilution: FLOW 1:100 - 1:250
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
Concentration
0.5 mg/mL by absorbance = 82.0 at 565 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed Description

Background
Anti-Mouse IgG (H&L) Phycoerythrin conjugated antibody generated in goat detects specifically mouse IgG (H&L). This secondary conjugated antibody anti-mouse is ideal for investigators who routinely perform immunomicroscopy and flow cytometry or FACS analysis as well as other antibody-based fluorescent assays requiring extremely low background levels.
Synonyms
Mouse IgG (H&L) Antibody, Gt-a-Mouse IgG(H&L) Phycoerythrin conjugated, Mouse IgG(H&L) Phycoerythrin Antibody in goat, Mouse IgG (H&L) Phycoerythrin conjugated Secondary Antibody.
Immunogen
Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Application Note
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, conjugation to phycoerythrin and subsequent purification, and extensive dialysis against the buffer stated above.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
R-Phycoerythrin (RPE)
Color
Red
Conjugation Reference
Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversible protein-protein conjugation. Biochem J 173, 723-737 (1978).
Molecular Weight
240000
Excitation Wavelength
488
Conjugation Chemistry
SATA/MBS