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Secondary Antibodies  >  Phycoerythrin Conjugated Antibodies
 

HUMAN IgG (H&L) (GOAT) Antibody Phycoerythrin conjugated (Min X Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins)

Goat Polyclonal
Code

609-108-123
Size

1 mL
Price

$200.00
Availability

Ships next business day.
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Shipping:
$40.00 to United States
Handling:
$35.00
Synonyms: Human Antibody, Gt-a-Human IgG, Human Antibody in Goat, Human Secondary Antibody

HUMAN IgG (H&L) (GOAT) Antibody Phycoerythrin conjugated (Min X Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins) Properties

Description
Anti-HUMAN IgG (H&L) (GOAT) Antibody Phycoerythrin conjugated (Min X Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins) - 609-108-123
Host
Goat
Specificity
IgG (H&L)
Target Species
Human
Clonality
Polyclonal
Application
IF Microscopy : 1:100 - 1:250
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Concentration
0.5 mg/mL by absorbance = 82.0 at 565 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Download CofA
Lot#  

HUMAN IgG (H&L) (GOAT) Antibody Phycoerythrin conjugated (Min X Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins) Description

Background
Anti-Human IgG whole molecule antibody generated in goat detects specifically human IgG whole molecule. This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Immunogen
Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in goat.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Human IgG and Human Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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AMCA Conjugates
Antigen Retrieval Methods
Azide Removal by Dialysis
Beta Galactosidase Conjugates
Biotin Binding Assay (HABA)
Biotin Conjugates
Cell Lysates Protocol
CyDye™ Conjugates
DyLight™ Antibody Conjugate Properties
DyLight™ Antibody Spectra
ELISA
ELISA to Quantify Collagen
Extended Conjugate Information Sheet
Fab Fragment Monovalent Antibodies
FACS / Flow Cytometry Analysis
Fluorescein Conjugates
Gel Shift Assay
General Antibody Information
Histone Immunoblotting
Immunoblotting
Immunocytochemistry
Immunofluorescence Microscopy
Immunofluorescence Microscopy - Assay Formats
Immunofluorescence Microscopy - ATTO-TEC
Immunofluorescence Microscopy - CyDye®
Immunofluorescence Microscopy - DyLight™
Immunofluorescence Microscopy - Fluorescent Labels
Immunofluorescence Microscopy - IRDye®
Immunofluorescence Microscopy - long
Immunofluorescence Microscopy - Microscope Types
Immunofluorescence Microscopy - Other Conjugates
Immunofluorescence Microscopy Protocol
Immunohistochemistry
Immunoprecipitation
Nuclear/Cytoplasmic Extract Isolation
Peroxidase & Alkaline Phosphatase
Phosphorylated State Proteins
Phosphorylated State Proteins - AKTs
Phosphorylated State Proteins - Long
Phosphorylated State Proteins - Pathways
Phosphorylated State Proteins - Phospho Specific
Phosphorylated State Proteins - Tailored Phosphorylated Target
Phosphorylated State Proteins - Western Blot
Phycoerythrin Conjugates
Protein A / Protein G Binding Reactivities
Recommended Dilutions
Rhodamine & Texas Red™
SDS-PAGE
Staining Paraffin Sections by PAP Procedure
USDA & Material Safety Notice
Western Blot - Buffers
Western Blot - Long
Western Blot - Preassembled Kits
Western Blot - Primary Antibody Reagents
Western Blot - Secondary Antibody Reagents
Western Blot - Substrates
Western Blot - Types of Blot Formats
Western Blot (Protein Immunoblot)
Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
R-Phycoerythrin (RPE)
Color
Red
Conjugation Reference
Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversible protein-protein conjugation. Biochem J 173, 723-737 (1978).
Molecular Weight
240000
Excitation Wavelength
488
Conjugation Chemistry
SATA/MBS