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Primary Antibodies  >  Phospho Specific Antibodies
 

Phosphoserine Antibody

Rabbit Polyclonal
Code

600-401-261
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Rockland's Affinity Purified anti-phosphoserine (pS) antibody is shown to detect phosphorylated proteins present in UV-treated mouse spleen cell lysates (top panel).
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Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: pS antibody, phospho serine AB, PhosphoSerine (pS) reactive antibody, proteinogenic amino acid antibodies, Phosphoserine [pS] polyclonal antibody, PhosphoSerine antibody produced in rabbit

Phosphoserine Antibody Properties

Description
Anti-PhosphoSerine (pS) [RABBIT) Antibody - 600-401-261
Host
Rabbit
Known Cross Reactivity
phosphoserine
Clonality
Polyclonal
Application
ELISA : 1:1,000-1:2,500
Western Blot : 1:500-1:1,000
Immunochemistry: User Optimized
ImmunoPrecipitation: 10 µg/mg protein sample
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
None
Stabilizer
50% (v/v) Glycerol
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Lot#  

Phosphoserine Antibody Description

Background
Anti-Phosphoserine Antibody detects phophorylated Serines. Serine is one of the proteinogenic amino acids. By virtue of the hydroxyl group, serine is classified as a polar amino acid. It is not essential to the human diet, since it is synthesized in the body from other metabolites, including glycine. Serine is important in metabolism in that it participates in the biosynthesis of purines and pyrimidines. It is the precursor to several amino acids including glycine and cysteine, and tryptophan in bacteria. It is also the precursor to numerous other metabolites, including sphingolipids and folate, which is the principal donor of one-carbon fragments in biosynthesis. Anti-phospho Serine Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Immunology, Cancer research and Signal Transduction research.
Immunogen
Phosphoserine Antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphoserine conjugated KLH.
Immunogen Type
Other
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Phosphoserine Antibody is suitable for use in ELISA, western blotting and immunoprecipitation assays.  The antibody reacts specifically with phosphoserine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphothreonine residues.  The antibody reacts with free phosphoserine, phosphoserine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphoserine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
Purity/Specifity
Anti-Phosphoserine Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated serine peptide coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.  Reactivity is specific for phosphoserine and minimal cross-reactivity is observed against phosphotyrosine or phosphothreonine.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry