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Primary Antibodies  >  Neuroscience Antibodies
 

Tamalin Antibody

Rabbit Polyclonal
Code

600-401-988
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's affinity purified anti-Tamalin to detect over-expressed Tamalin in HEK293 cells (lane 2, arrowhead). Lane 1 shows the non-transfected control. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:2,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
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Synonyms: DXImx47e antibody, GRASP 1 antibody, GRIP 1 associated protein 1 antibody, GRIP associated protein 1 antibody, GRIP1 associated protein 1 antibody, GRIPAP 1 antibody, General receptor for phosphoinositides 1-associated scaffold protein

Tamalin Antibody Properties

Description
Anti-Tamalin (RABBIT) Antibody - 600-401-988
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse
Clonality
Polyclonal
Application
ELISA : 1:4,000 - 1:20,000
Western Blot : 1:500 - 1:3,000
ImmunoPrecipitation: 1µg
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.03 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
GRASP
Download CofA
Lot#  

Tamalin Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Tamalin, also named General receptor for phosphoinositides 1-associated scaffold protein (GRASP) is a PDZ (post-synaptic density protein/Drosophila disc large tumor suppressor/zo-1) domain-containing protein that interacts with group 1 metabotropic glutamate receptors (mGluRs).  The PDZ domain-containing amino-terminal half of Tamalin binds directly to the class I PDZ-binding motif of group 1 mGluRs.  The carboxyl-terminal half of Tamalin binds to cytohesins, which are guanine nucleotide exchange factors (GEFs) specific for the ADP-ribosylation factor (ARF) family of small GTP-binding proteins.  Tamalin forms a protein complex with group 1 mGluRs at the post-synaptic site of specific neuronal cells and serves as a key scaffold protein that links a complex formation between mGluR1a and cytohesins.  It is reported that Tamalin plays a key role in the association of group 1 mGluRs with the ARF-specific GEF proteins and contributes to intracellular trafficking and the macromolecular organization of group 1 mGluRs at synapses.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse Tamalin protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, western blotting, and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 42 kDa in size corresponding to Tamalin protein by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity purified antibody is directed against mouse Tamalin protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest cross-reactivity with Tamalin protein from rat based on a 94% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Kitano J., et al. (2002) Tamalin, a PDZ Domain-Containing Protein, Links a Protein Complex Formation of Group 1 Metabotropic Glutamate Receptors and the Guanine Nucleotide Exchange Factor Cytohesins. J. Neurosci. 22(4):1280–1289. Nevrivy D. J., et al. (2000)  Interaction of GRASP, a protein encoded by a novel retinoic acid-induced gene, with members of the cytohesin family of guanine nucleotide exchange factors. J. Biol. Chem. 275: 16827-16836. Kimura K., et al. (2004) Hyperpolarization-activated, cyclic nucleotide-gated HCN2 cation channel forms a protein assembly with multiple neuronal scaffold proteins in distinct modes of protein-protein interaction. Genes Cells 9(7):631-640. Hirose M., et al. (2004) Phosphorylation and recruitment of Syk by immunoreceptor tyrosine-based activation motif-based phosphorylation of tamalin. J. Biol. Chem. 279(31):32308-32315. Kitano J., et al. (2003) Tamalin is a scaffold protein that interacts with multiple neuronal proteins in distinct modes of protein-protein association. J. Biol. Chem. 278(17):14762-14768.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mouse, human
Catalog Number
100-401-223

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
mouse
Catalog Number
100-401-225

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail
Catalog Number
600-401-694

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mouse
Catalog Number
600-401-695

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry