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Primary Antibodies  >  Stem Cell Antibodies
 

Jagged-2 Antibody

Rabbit Polyclonal
Code

600-401-852
Size

100 µg
Price

$294.00
Availability

Ships next business day.
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$85.00 to United States
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Synonyms: D12Ggc2e antibody, HJ 2 antibody, HJ2 antibody, JAG 2 antibody, JAG2 antibody, Jagged 2 precursor antibody, Jagged2 antibody, Syndactylism antibody, Protein jagged-2, SER2, hJ2

Jagged-2 Antibody Properties

Description
Anti-Jagged-2 (RABBIT) Antibody - 600-401-852
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:500- 1:2,000
Other Dilution: User Optimized
Species of Origin
Rabbit
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.9 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
Jag2
Download CofA
Lot#  

Jagged-2 Antibody Description

Background
Jagged 2 antibdody detect the Jagged protein. Jagged 2 is a Notch ligand, which shows distinct expression patterns in the developing and adult brain that overlaps that of Notch1. The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. The protein encoded by this gene is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for this gene. Anti-Jagged 2 antibody is ideal for investigators involved in developmental biology research.
Immunogen
Anti-Jagged-2 [RABBIT) Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 111-128 of Mouse Jagged-2.  Jagged 2 is a Notch ligand, which shows distinct expression patterns in the developing and adult brain that overlaps that of Notch1.  Swiss Prot accession Q9QYE5. Expected to cross-react with rat Jagged-2.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Jagged-2 Antibody has been tested for use in ELISA against the immunizing peptide.  Reactivity in other immunoassays including western blot, immunohistochemistry, and immunofluorescence is expected.
Purity/Specifity
Anti-Jagged-2 Antibody produced repeated immunizations with a synthetic peptide corresponding to residues near the middle of the mouse Jagged 2 protein and purified by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Luo B, Aster JC, Hasserjian RP, Kuo F, Sklar J (October 1997). "Isolation and functional analysis of a cDNA for human Jagged2, a gene encoding a ligand for the Notch1 receptor". Mol Cell Biol 17 (10): 6057–67.
Specific Reference
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AMCA Conjugates
Antigen Retrieval Methods
Azide Removal by Dialysis
Beta Galactosidase Conjugates
Biotin Binding Assay (HABA)
Biotin Conjugates
Cell Lysates Protocol
CyDye™ Conjugates
DyLight™ Antibody Conjugate Properties
DyLight™ Antibody Spectra
ELISA
ELISA to Quantify Collagen
Extended Conjugate Information Sheet
Fab Fragment Monovalent Antibodies
FACS / Flow Cytometry Analysis
Fluorescein Conjugates
Gel Shift Assay
General Antibody Information
Histone Immunoblotting
Immunoblotting
Immunocytochemistry
Immunofluorescence Microscopy
Immunofluorescence Microscopy - Assay Formats
Immunofluorescence Microscopy - ATTO-TEC
Immunofluorescence Microscopy - CyDye®
Immunofluorescence Microscopy - DyLight™
Immunofluorescence Microscopy - Fluorescent Labels
Immunofluorescence Microscopy - IRDye®
Immunofluorescence Microscopy - long
Immunofluorescence Microscopy - Microscope Types
Immunofluorescence Microscopy - Other Conjugates
Immunofluorescence Microscopy Protocol
Immunohistochemistry
Immunoprecipitation
Nuclear/Cytoplasmic Extract Isolation
Peroxidase & Alkaline Phosphatase
Phosphorylated State Proteins
Phosphorylated State Proteins - AKTs
Phosphorylated State Proteins - Long
Phosphorylated State Proteins - Pathways
Phosphorylated State Proteins - Phospho Specific
Phosphorylated State Proteins - Tailored Phosphorylated Target
Phosphorylated State Proteins - Western Blot
Phycoerythrin Conjugates
Protein A / Protein G Binding Reactivities
Recommended Dilutions
Rhodamine & Texas Red™
SDS-PAGE
Staining Paraffin Sections by PAP Procedure
USDA & Material Safety Notice
Western Blot - Buffers
Western Blot - Long
Western Blot - Preassembled Kits
Western Blot - Primary Antibody Reagents
Western Blot - Secondary Antibody Reagents
Western Blot - Substrates
Western Blot - Types of Blot Formats
Western Blot (Protein Immunoblot)
Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-405

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, dog, chimpanzee
Catalog Number
100-401-406

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
100-401-407

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-408

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry