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Primary Antibodies  >  Stem Cell Antibodies
 

Nestin Antibody

Rabbit Polyclonal
Code

600-401-417
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Immunohistochemistry with Anti-nestin antibody
Tissue:   mouse kidney (Left) and human kidney (Right). 
Fixation: formalin-fixed, paraffin-embedded tissue
Antigen retrieval: heat-induced 
Primary antibody: 5 µg/ml
Staining:  antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
Western blot using Rockland's Affinity Purified anti-Nestin antibody shows detection of a band ~220 kDa corresponding to mouse Nestin (arrowhead).  Approximately 30 µg of MEF whole cell lysate was separated by SDS-PAGE using a 4-20% gradient gel.  After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2,000 overnight at 4°C.  The membrane was then washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.
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Synonyms: nestin, ESTM 46 antibody, FLJ 21841 antibody, FLJ21841 antibody, Intermediate filament protein antibody

Nestin Antibody Properties

Description
Anti-Nestin (RABBIT) Antibody - 600-401-417
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:80,000
Western Blot : 1:500 - 1:3,000
Immunochemistry: 5ug/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
NES
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Lot#  

Nestin Antibody Description

Background
Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985).  Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube.  Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells.  Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.  Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Immunogen
Anti-Nestin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store anti-Nestin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Affinity purified Anti-Nestin has been tested for use in ELISA, Immunohistochemistry (IHC) and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 200-220 kDa in size corresponding to Nestin protein by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
Nestin antibody is directed against human Nestin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross reactivity with Nestin protein from human and macaque (100% homology).  Based on protein sequence homology also expect partial reactivity against Nestin homologues from rabbit (94%), mouse (reactivity shown above), rat and hamster (88%), and bovine and dog (82%).  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Dahlstrand,J., Zimmerman,L.B., McKay,R.D. and Lendahl,U. (1992) Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments. J. Cell. Sci. 103 (Pt 2), 589-597. Yaworsky,P.J. and Kappen,C. (1999) Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene. Dev. Biol. 205 (2), 309-321. Hockfield,S. and McKay,R.D. (1985) Identification of major cell classes in the developing mammalian nervous system. J. Neurosci. 5 (12), 3310-3328. Mokry,J., Cizkova,D., Filip,S., Ehrmann,J., Osterreicher,J., Kolar,Z. and English,D. (2004) Nestin expression by newly formed human blood vessels. Acta Neurochir. Suppl. 13 (6), 658-664. Aihara,M., Sugawara,K., Torii,S., Hosaka,M., Kurihara,H., Saito,N. and Takeuchi,T. (2004) Angiogenic endothelium-specific nestin expression is enhanced by the first intron of the nestin gene. Lab. Invest. 84 (12), 1581-1592.
Specific Reference
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Product Type
Controls;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat. chicken, bovine
Catalog Number
200-301-880

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat. chicken, bovine
Catalog Number
600-401-880

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
Mouse, Rat, Human, Monkey, Sheep, Bovine, Goat, Guinea Pig, Swine, Hamster
Catalog Number
600-401-886

Product Type
Controls;
Applications
ELISA, WB, IHC
Reacts With
Mouse, Rat, Human, Monkey, Sheep, Bovine, Goat, Guinea Pig, Swine, Hamster
Catalog Number
600-403-886

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry