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Controls  >  Loading Control Antibodies
 

GAPDH Antibody

Rabbit Polyclonal
Code

600-401-A33
Size

100 µg
Price

$265.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Immunohistochemical staining using Rockland's Affinity Purified anti-GAPDH antibody at 10 µg/ml shows detection of GAPDH in human liver tissue.  Tissue was fixed in 4% paraformaldehyde using a section 4.2 µm thick.
Western blot using Rockland's Affinity Purified anti-GAPDH antibody shows detection of a single band at ~39 kDa corresponding to GAPDH (arrowhead) in HeLa cell lysates.  The predicted MW of GAPDH is 36 kDa.  GAPDH was detected using 125 ng/ml (lane A), 250 ng/ml (lane B) and 500 ng/ml (lane C) concentrations of primary antibody.
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Synonyms: Loading Control Antibody, Anti-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD

GAPDH Antibody Properties

Description
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (RABBIT) Antibody - 600-401-A33
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Mouse, Human, Rat, Rabbit, Chicken, Bovine, Swine
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:40,000
IF Microscopy : 1:100 - 1:1,000
Western Blot : 1:1,000 - 1:5,000
Immunohistochemistry: 1:100 - 1:1,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
GAPDH
Related Links
Download CofA
Lot#  

GAPDH Antibody Description

Background
GAPDH loading control antibody is ideal for Western Blotting, ELISA, IHC and IF Microscopy. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control antibody in immunoblots. A loading control antibody is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease. Anti-GAPDH antibody is ideal for investigators involved in apoptosis, cancer, DNA damage and repair and neuroscience.
Immunogen
GAPDH Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH).
Storage Condition
Store Anti-GAPDH antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-GAPDH Antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation.  Expect a band at ~36 kDa in size corresponding to GAPDH by western blotting in the appropriate cell lysate or extract.  GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control marker.
Purity/Specifity
Anti-GAPDH Antibody is directed against human GAPDH protein.  The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest that this antibody would react with GAPDH from a wide range of organisms, including most vertebrates and some yeast.  Broad reactivity makes this antibody an excellent loading control.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Sirover,M.A. (2005) New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 95:45-52. Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. (2005); 45:269-290. Raje,C.I., Kumar,S., Harle,A., Nanda,J.S. and Raje,M. (2007) The macrophage cell surface glyceraldehyde-3-phosphate dehydrogenase is a novel transferrin receptor. J. Biol. Chem. 282 (5):3252-3261.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry