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Primary Antibodies  >  Loading Control Antibodies
 

beta Actin Antibody

Rabbit Polyclonal
NCI Collaboration
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Western Blot of Rabbit Anti-Beta Actin Antibody.  
Lane 1:  molecular weight.  
Lane 2:  human embryonic kidney 293.  
Lane 3:  human lung carcinoma A549.  
Lane 4:  mouse brain.  
Load:  35 µg per lane.
Primary antibody:  Beta Actin antibody at 1:1,500 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  ~42 kDa corresponding to beta Actin (arrowhead). 
Other band(s): none.
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Number:
600-401-886
Size:
200 µg
Price:
$265.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

beta Actin Antibody Properties

Description
Anti-beta Actin (RABBIT) Antibody - 600-401-886
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Mouse, Rat, Human, Monkey, Sheep, Bovine, Goat, Guinea Pig, Swine, Hamster
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:40,000
IF Microscopy : 1:500 - 1:2,000
Western Blot : 1:1,000 - 1:4,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
ACTB
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beta Actin Antibody Description

Background
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Synonyms
Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody
Immunogen
beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 359-368 of Human beta Actin.
Immunogen Type
Peptide
Storage Condition
Store beta-Actin Loading Control Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-beta Actin Antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
Anti-beta-Actin Loading Control Antibody reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein.  A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Hu,P., Wu,S. and Hernandez,N. (2004) A role for beta-actin in RNA polymerase III transcription. Genes Dev. 18 (24), 3010-3015. Dahlen,A., et al. (2004) Activation of the GLI oncogene through fusion with the beta-actin gene (ACTB) in a group of distinctive pericytic neoplasms: pericytoma with t(7;12). Am. J. Pathol. 164 (5), 1645-1653. Navarro-Lerida,I., Martinez Moreno,M., Roncal,F., Gavilanes,F., Albar,J.P. and Rodriguez-Crespo,I. (2004) Proteomic identification of brain proteins that interact with dynein light chain LC8. Proteomics 4 (2), 339-346.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry