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Primary Antibodies > Stem Cell Antibodies

SKG-1 Antibody

Rabbit Polyclonal

Code

200-401-844

Size

500 µg

Price

$294.00

Availability

Ships next business day.

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Rockland's Affinity Purified anti-SGK-1 antibody was used at a 15 µg/ml to detect nuclear and cytoplasmic signal in a variety of tissues including adrenal, heart, liver, ovary, pancreas, placenta, skin, spleen, testes, thyroid and uterus. Low to moderate levels of background staining were noted. This image shows SGK-1 staining of human pancreas. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

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Synonyms: Serum/glucocorticoid-regulated kinase 1, SGK1 antibody, Cellular stress response antibodies, Serine/threonine-protein kinase Sgk1

Datasheet
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SKG-1 Antibody Properties

Description

Anti-Human SGK-1 (Rabbit) - 200-401-844

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human, mouse, rat

Clonality

Polyclonal

Application

ELISA : 1:4,000 - 1:16,000
Western Blot : 1:500 - 1:3,000
Immunohistochemistry: 10 µg/ml to 20 µg/ml

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

5.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

SGK1

SKG-1 Antibody Description

Background

SGK-1 (also called Serine/threonine-protein kinase Sgk1 and Serum/glucocorticoid-regulated kinase 1) is a protein kinase that plays an important role in cellular stress response. SGK1 activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. This kinase mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A and phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, Kv1.3, or EAAT1. SGK1 is localized to the cytoplasm and upon phosphorylation is translocated to the nucleus. The kinase is expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Induction occurs upon exposure to glucocorticoids and by excessive extracellular glucose or TGF-beta, in cultured cells. SGK-1 is regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1. The kinase is ubiquitinated by NEDD4L; which promotes proteasomal degradation.

Immunogen

SKG-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 419-431 of human SGK -1.

Immunogen Type

Peptide

Storage Condition

Store SKG-1 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

SKG-1 Antibody antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~49 kDa in size corresponding to SGK-1 by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

Anti-SKG-1 Antibody is directed against human SGK-1 protein. The product was protein A purified from monospecific antiserum. A BLAST analysis was used to suggest reactivity with this protein from human, macaque and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with SGK-1 homologues from mouse, rat, dog, chicken, bovine, rabbit and spiny dogfish based on an 92% sequence homology for the immunogen (12/13) from these sources. Cross reactivity with SGK-1 homologues from other sources has not been determined.

General Reference

Waldegger,S., Barth,P., Raber,G. and Lang,F. (1997) Cloning and characterization of a putative human serine/threonine protein kinase transcriptionally modified during anisotonic and isotonic alterations of cell volume. Proc. Natl. Acad. Sci. U.S.A. 94 (9), 4440-4445. Waldegger,S., Erdel,M., Nagl,U.O., Barth,P., Raber,G., Steuer,S., Utermann,G., Paulmichl,M. and Lang,F. (1998) Genomic organization and chromosomal localization of the human SGK protein kinase gene. Genomics 51 (2), 299-302. Kobayashi,T., Deak,M., Morrice,N. and Cohen,P. (1999) Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase. Biochem. J. 344 PT 1, 189-197.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra