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Supporting Reagents > Enzyme Substrates

TMB ELISA Peroxidase Substrate

Code

TMBE-100

Size

100 mL

Price

$90.00

Availability

Ships next business day.

Order Offline:

Phone: 800.656.7625

E-mail: orders@rockland-inc.com

Fax: 610.367.7825

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Rockland Immunochemicals produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabalizer (p/n MB-063-0100) added to the microwell plate and incubated overnight at 4°C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n MB-064-1000) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n MB-066-0100), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer (p/n MB-060-0100), added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB ELISA Peroxidase Substrate (p/n TMBE-1000) was added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm.

Shipping & Handling Fees Apply.

Shipping:

$40.00 to United States

Handling:

$35.00

Synonyms: 3,3’,5,5’-Tetramethylbenzidine; TMB-E; TMBE

Datasheet
References
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TMB ELISA Peroxidase Substrate Properties

Description

TMB ELISA PEROXIDASE SUBSTRATE - TMBE-100

Application

ELISA : 1x

Substrate/Enzyme Type

Substrate

Product Type

Substrate

Physical State

Liquid - clear to very light blue colored liquid

Shipping condition

Ambient

Label

Unconjugated

Stabilizer

Proprietary buffer with enhancer

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TMB ELISA Peroxidase Substrate Description

Background

TMB ELISA Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. This reaction can be halted with acid to change the TMB to yellow. TMB Elisa Peroxidase Substrate is ideal for investigators in Microbiology, Immunology, and Cancer research.

Storage Condition

Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.

Application Note

TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB ELISA Peroxidase Substrate comes ready to use.

Purity/Specifity

pH: 4.0 +/- 0.2 Stability @ 18° to 26°C: PASS Stability @ 4°C: PASS QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per ELISA: PASS

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

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