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Anti-NCOA3 (RABBIT) Antibody - 600-401-A40S

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Primary Antibodies > Enzyme Antibodies

NCOA3 Antibody

Rabbit Polyclonal

Code

600-401-A40

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's affinity purified anti-NCOA3 antibody shows detection of NCOA3 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kDa, indicated by the arrowhead, corresponds to NCOA3. Mouse NCOA3 (lanes 1 and 4) appear as ~105 kDa bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Personal communication, H. Ying, NCI, Bethesda, MD.

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Synonyms: Nuclear receptor coactivator 3 NCoA-3 Thyroid hormone receptor activator molecule 1 TRAM-1, pCIP, bHLHe42 Receptor-associated coactivator 3, RAC-3 Amplified in breast cancer 1 protein, AIB-1 Steroid receptor coactivator protein 3, SRC-3

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NCOA3 Antibody Properties

Description

Anti-NCOA3 (RABBIT) Antibody - 600-401-A40

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:175,000
Western Blot : 1:500 - 1:1,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.24 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

NCOA3

NCOA3 Antibody Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The NCOA3 (also known as nuclear receptor coactivator 3 isoform a) is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions in a hormone-dependent fashion. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. NCOA3 probably acts via remodeling of chromatin and is involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs),

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human NCOA3 isoform a protein.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting.

Purity/Specifity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with several isoforms of human NCOA3 protein (a, b, d, e and f). A BLAST analysis was used to suggest cross-reactivity with NCOA3 from human and macaque sources based on a 100% homology with the immunizing sequence. Expect partial reactivity with NCOA3 from dog, bovine, mouse and rat sources based on partial sequence homology. Cross-reactivity with NCOA3 from other sources has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Li,H.J., Haque,Z., Lu,Q., Li,L., Karas,R. and Mendelsohn,M.(2007) Steroid receptor coactivator 3 is a coactivator for myocardin, the regulator of smooth muscle transcription and differentiation. Proc. Natl. Acad. Sci. U.S.A. 104 (10), 4065-4070. Naeem,H., Cheng,D., Zhao,Q., Underhill,C., Tini,M., Bedford,M.T. and Torchia,J. (2007) The activity and stability of the transcriptional coactivator p/CIP/SRC-3 are regulated by CARM1-dependent methylation. Mol. Cell. Biol. 27 (1), 120-134. Hossain,A., Kuo,M.T. and Saunders,G.F. (2006) Mir-17-5p regulates breast cancer cell proliferation by inhibiting translation of AIB1 mRNA. Mol. Cell. Biol. 26 (21), 8191-8201.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra