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Primary Antibodies  >  Enzyme Antibodies
 

Selenophosphate Synthetase 1 Antibody

Rabbit Polyclonal
Code

100-401-A22
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's anti-SPS1 antibody shows detection of endogenous SPS1 in NIH3T3 cell.  No signal is seen in lysates from cells after pre-treatment with SPS1 siRNA.  Endogenous SPS1 can be detected in mouse kidney and liver tissue lysates.  Negligible cross-reactivity is seen against recombinant SPS2.  The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
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Synonyms: Selenide, water dikinase 1 EC=2.7.9.3 Selenophosphate synthetase 1 Selenium donor protein 1

Selenophosphate Synthetase 1 Antibody Properties

Description
Anti-Selenophosphate Synthetase 1 (SPS1) (RABBIT) Antibody - 100-401-A22
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse, human, rat
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:500 - 1:2,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SEPHS1, Sps1
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Lot#  

Selenophosphate Synthetase 1 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Selenophosphate synthetase 1 (SPS1) (also known as selinide water dikinase or selenium donor protein 1) is the homolog of the SelD gene product in E. coli.  In E. coli, SelD generates the selenium donor for selenocysteine tRNA biosynthesis.  The function of SPS1 in mammals is still unknown but appears to differ from that of E. coli SelD.
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS1.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
This antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 43 kDa in size corresponding to SPS1 by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This antibody is directed against mouse SPS1.  The product is delipidated and defibrinated antiserum.  A BLAST analysis was used to suggest cross-reactivity with SPS1 from mouse, human and rat based on a 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Small-Howard A, Morozova N, Stoytcheva Z, Forry EP, Mansell JB, Harney JW, Carlson BA, Xu XM, Hatfield DL, Berry MJ (2006) Supramolecular complexes mediate selenocysteine incorporation in vivo.  Mol. Cell Biol. 6:2337-46. Ogasawara Y, Lacourciere GM, Ishii K, Stadtman TC (2005) Characterization of potential selenium-binding proteins in the selenophosphate synthetase system.  Proc. Natl Acad. Sci. USA 102(4):1012-6. Stadtman T  (2004) Methanococcus vannielii selenium metabolism: purification and N-terminal amino acid sequences of a novel selenium-binding protein and selenocysteine lyase. IUBMB Life 56(7):427-31. Tamura T, Yamamoto S, Takahata M, Sakaguchi H, Tanaka H, Stadtman TC, Inagaki K (2004) Selenophosphate synthetase genes from lung adenocarcinoma cells: Sps1 for recycling L-selenocysteine and Sps2 for selenite assimilation. Proc. Natl Acad. Sci. USA 101(46):16162-7.
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry