House Secondary Banner
 
You may also want to consider these related products.
Anti-Erk2 (RABBIT) Antibody - 100-401-218
Anti-Erk2 (RABBIT) Antibody - 100-401-218
Anti-Human Sprouty-2 (RABBIT) Antibody - 600-401-241
Anti-Human Sprouty-2 (RABBIT) Antibody - 600-401-241
Anti-p44 MAP Kinase (ERK1) [RABBIT) Antibody - 600-401-943
Anti-p44 MAP Kinase (ERK1) [RABBIT) Antibody - 600-401-943
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Primary Antibodies  >  Stem Cell Antibodies
 

Sprouty-4 Antibody

Rabbit Polyclonal
Code

600-401-697
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Immunohistochemistry of Rabbit anti-Sprouty-4 antibody.  
Tissue:  human heart tissue.  
Fixation:  formalin fixed paraffin embedded.  
Antigen retrieval:  not required.
Primary antibody:  Sprouty-4 antibody at 2.5 µg/ml for 1 h at RT.
Secondary antibody:  Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.
Localization:  Sprouty-4 is cytoplasmic.
Staining:  Sprouty-4 as precipitated red signal with hematoxylin purple nuclear counterstain.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Sprouty-4, SPRY4

Sprouty-4 Antibody Properties

Description
Anti-Sprouty-4 (RABBIT) Antibody - 600-401-697
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, chimpanzee
Clonality
Polyclonal
Application
ELISA : 1:3,000 - 1:12,000
Western Blot : 1:100 - 1:1,000
Immunochemistry: 1 µg/ml to 10 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.1 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SPRY4
Download CofA
Lot#  

Sprouty-4 Antibody Description

Background
Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.  SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 306-322 of Human Sprouty-4 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band (maybe a doublet) ~35 kDa in size corresponding to Sprouty-4 by western blotting in the appropriate cell lysate or extract.  Some non-specific banding may be observed using this antibody.
Purity/Specifity
This affinity purified antibody is directed against human Sprouty-4 protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity is expected with Sprouty-4
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Ding,W., Bellusci,S., Shi,W. and Warburton,D. (2004) Genomic structure and promoter characterization of the human Sprouty4 gene, a novel regulator of lung morphogenesis. Am. J. Physiol. Lung Cell Mol. Physiol. 287 (1), L52-L59. Sasaki,A., Taketomi,T., Kato,R., Saeki,K., Nonami,A., Sasaki,M., Kuriyama,M., Saito,N., Shibuya,M. and Yoshimura,A. (2003) Mammalian Sprouty4 suppresses Ras-independent ERK activation by binding to Raf1. Nat. Cell Biol. 5 (5), 427-432. Leeksma,O.C., Van Achterberg,T.A., Tsumura,Y., Toshima,J., Eldering,E., Kroes,W.G., Mellink,C., Spaargaren,M., Mizuno,K., Pannekoek,H. and de Vries,C.J. (2002) Human sprouty 4, a new ras antagonist on 5q31, interacts with the dual specificity kinase TESK1. Eur. J. Biochem. 269 (10), 2546-2556.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-218

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
600-401-241

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human
Catalog Number
600-401-943

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry