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Primary Antibodies  >  Chromatin Antibodies
 

CENP-Q Antibody

Rabbit Polyclonal
Code

200-401-B48
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Immunofluorescence microscopy using Rockland's protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENP-Q signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP-Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis.  Nuclei are counter stained with bisbenzimide.  Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD
Western blot using Rockland's protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in a HeLa whole cell lysate (lane 1, arrowhead).   The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5?g/mL, followed by washes and incubation with to the secondary antibody.   Lane 1:  Lysates from HeLa cells transfected with control sh-virus.  Lane 2:  Lysates from HeLa cells transfected with Cenp-Q sh-virus.  Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
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Synonyms: Cenp-Q, centromere protein Q, CENPQ

CENP-Q Antibody Properties

Description
Anti-CENP-Q (RABBIT) Antibody - 200-401-B48
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:5,000 1:20,000
Western Blot : 1:100 - 1:500
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.15 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CENPQ
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Lot#  

CENP-Q Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cenp-Q (also known as centromere protein Q or CENPQ) is a nuclear/centromeric protein that is one of the critical components that constitutes the CENP-O complex at the kinetochores and appears to stabilize PBIP1/CENP-U(50)/MLF1IP in the complex. This complex is important for proper recruitment of polo-like kinase 1 (Plk1) to the mitotic kinetochores. A failure in this process results in improper microtubule attachment to the kinetochores and chromosome missegregation that ultimately lead to aneuploidy.
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length human CENP-Q recombinant protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein A purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26-31 kDa in size corresponding to human CENP-Q by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human CENP-Q protein. A BLAST analysis of the full length sequence was used to suggest partial cross-reactivity with CENP-Q based on the following percentage homologies: macaque (91%), horse (76%), bovine (74%), dog (73%), swine (71%), mouse (65%) and rat (60%). Cross-reactivity with CENP-Q from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Hori T, Okada M, Maenaka K, Fukagawa T. (2008) CENP-O Class Proteins Form a Stable Complex and Are Required for Proper Kinetochore Function. Mol Biol Cell. Mar;19(3):843-54. Epub 2007 Dec 19. Okada M, Cheeseman IM, Hori T, Okawa K, McLeod IX, Yates JR 3rd, Desai A, Fukagawa T. (2006) The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. Nat Cell Biol. May;8(5):446-57. Epub 2006 Apr 16. Foltz DR, Jansen LE, Black BE, Bailey AO, Yates JR 3rd, Cleveland DW. (2006) The human CENP-A centromeric nucleosome-associated complex. Nat Cell Biol. May;8(5):458-69. Epub 2006 Apr 16.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IF, IP
Reacts With
human
Catalog Number
200-301-901

Product Type
Secondary Antibodies;
Applications
FLISA, IF, FC
Catalog Number
211-1202

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-446

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry