This Chemiluminescent Western Blot Kit allows for the detection of primary human polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
Because of the extremely high sensitivity of our FemtoMax™Chemiluminescent Substrate Kit for Western Blotting, primary and secondary antibodies can be used at greater dilutions. If you currently dilute your primary antibody 1:1,000 using an ECL substrate equivalent, then dilute your antibody 1:5,000 using the FemtoMax? substrate.