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Primary Antibodies  >  Sample Size Primary Antibodies
 

HICE1 Antibody

Rabbit Polyclonal
Code

600-401-C53S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
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Western Blot of Rabbit Anti-Hice1 antibody.  
Lane 1:  HeLa cell extracts of untransfected cells (-).  
Lane 2:  transfected HeLa cell extracts with Flag X3-Hice1 WT (WT).
Lane 3:  transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A).    
Load:  35 µg per lane.
Primary antibody:  Hice1 antibody at 0.5 µg/mL for overnight at 4°C.
Secondary antibody:  IRDye800™ Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  44.8 kDa, 48 kDa for Hice1. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: HAUS8, HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48

HICE1 Antibody Properties

Description
Anti-Hice1 (Rabbit) Antibody - 600-401-C53S
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:30,000-1:45,000
Western Blot : 0.5 µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.06 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
Hice1
Download CofA
Lot#  

HICE1 Antibody Description

Background
Anti-Hice1 is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the ?TuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.
Immunogen
Anti-Hice1 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region of amino acids containing serine 70 of human Hice1.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
Hice1 antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kDa in size corresponding to human Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Purity/Specifity
Anti-Hice1 affinity purified antibody is directed against human Hice1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Wu G., Lin Y., Wei R., Chen Y., Shan Z., Lee W., (2008) Hice1, a Novel Microtubule-Associated Protein Required for Maintenance of Spindle Integrity and Chromosomal Stability in Human Cells. Mol Cell Biol. Jun;28(11):3652-62. Uehara R., Nozawa R., Tomioka A., Petry S., Vale D., Obuse C., Goshima G., (2009) The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells. Proc Natl Acad Sci U S A. Apr 14.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Human
Catalog Number
109-401-C39

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
600-401-C52


Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry