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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

HICE1 PS70 Antibody

Rabbit Polyclonal
Code

600-401-C52
Size

100 µg
Price

$279.00
Availability

Ships next business day.
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Synonyms: HAUS8, HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48

HICE1 PS70 Antibody Properties

Description
Anti-Hice1 pS70 (Rabbit) Antibody - 600-401-C52
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:17,000-1:45,000
Western Blot : 1.22 µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.22 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
Hice1
Download CofA
Lot#  

HICE1 PS70 Antibody Description

Background
Hice1 pS70 is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the ?TuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.
Immunogen
Anti-Hice1 pS70 Antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 70 of Hice1.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Hice1 pS70 antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kDa in size corresponding to human phosphorylated Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Purity/Specifity
Hice1 pS70 affinity purified antibody is directed against the phosphorylated form of human Hice1 protein at the S70 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.  The antibody is specific for the phosphorylated form of Hice1.   Reactivity with non-phosphorylated human Hice1 is minimal by ELISA and western blot.  A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Wu G., Lin Y., Wei R., Chen Y., Shan Z., Lee W., (2008) Hice1, a Novel Microtubule-Associated Protein Required for Maintenance of Spindle Integrity and Chromosomal Stability in Human Cells. Mol Cell Biol. Jun;28(11):3652-62. Uehara R., Nozawa R., Tomioka A., Petry S., Vale D., Obuse C., Goshima G., (2009) The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells. Proc Natl Acad Sci U S A. Apr 14.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Human
Catalog Number
109-401-C39

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
600-401-C53


Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry