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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

CDK9 phospho T29 Antibody

Rabbit Polyclonal
Code

600-401-996
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's affinity purified anti-CDK9 pT29 antibody shows detection of phosphorylated CDK9. 100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29.  The primary antibody was used at a 1:1000 dilution. Personal Communication, J. Brady, NCI, Bethesda, MD.
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Synonyms: Cell division protein kinase 9 Cyclin-dependent kinase 9 Serine/threonine-protein kinase PITALRE Cell division cycle 2-like protein kinase 4 C-2K

CDK9 phospho T29 Antibody Properties

Description
Anti-Cyclin-Dependent Kinase 9 (CDK9) pT29 [RABBIT] Antibody - 600-401-996
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:24,000
Western Blot : 1:200 - 1:2,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.3 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CDK9
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Lot#  

CDK9 phospho T29 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family.  CDK family members are highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2 and are known as important cell cycle regulators.  CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2.  This kinase was also found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II.  This protein forms a complex with, and is regulated by, its regulatory subunit, cyclin T or cyclin K. HIV-1 Tat protein
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T29 in the human CDK9 protein.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 42 kDa in size corresponding to phosphorylated CDK9 protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with human CDK9 pT29 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide.  This antibody is specific for human CDK9 protein phosphorylated at T29.  A BLAST analysis was used to suggest cross-reactivity with CDK9 from human, mouse and rat based on 100% homology with the immunizing sequence.  Cross-reactivity with CDK9 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Zhou, M., Lu, H., Park, H., Wilson-Chiru, J., Linton, R. and Brady, J.N. (2006) Tax Interacts with P-TEFb in a Novel Manner To Stimulate Human T-Lymphotronic Virus Type 1 Transcription. J. Virol. 80 (10): 4781-4791. Jang, M.K., Mochizuki, K., Zhou, M., Jeong, H.-S., Brady, J.N. and Ozato, K. (2005) Bromodomain Protein Brd4 is a Positive Regulatory Component of P-TEFb and Stimulates RNA Polymerase II Dependent Transcription. Mol. Cell 19: 523-534. Liu, H. and Herrmann, C.H. (2005) Differential localization and expression of the Cdk9 42k and 55k isoforms.  J. Cell. Physiol. 203 (1): 251-260. Peng, J., Zhu, Y., Milton, J.T. and Price, D.H. (1998). Identification of multiple cyclin subunits of human P-TEFb. Genes Dev. 12 (5): 755-762. Zhou, Q., Chen, D., Pierstorff, E. and Luo, K. (1998)  Transcription elongation factor P-TEFb mediates Tat activation of HIV-1 transcription at multiple stages.  EMBO J. 17(13): 3681-3691.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, mouse, rat
Catalog Number
100-401-158

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-161

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-162

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-163

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry