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Primary Antibodies  >  Cancer Research Antibodies
 

MACC1 Antibody

Rabbit Polyclonal
Code

600-401-C07
Size

100 µg
Price

$309.00
Availability

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Immunohistochemistry showing detection of MACC1 in human liver tissue.  A-MACC1 antibody was used at 2.5 µg/mL.
Western blot showing detection of MACC1 protein in mouse liver tissue lysate.  A-MACC1 was used at 1 µg/mL (lane A) and at 2 µg/mL (lane B).
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Synonyms: MACC1 (IN), Metastasis associated in colon cancer 1, SH3 domain-containing protein 7a5

MACC1 Antibody Properties

Description
Anti-MACC1 (Rabbit) Antibody - 600-401-C07
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:40,000
Western Blot : 1.0 - 2.0 µg/mL
Immunochemistry: 2.5 µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
MACC1
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Lot#  

MACC1 Antibody Description

Background
Metastasis associated in colon cancer 1 (MACC1) is a key regulator of the hepatocyte growth factor (HGF)-HGF receptor (MET) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. MACC1 protein consists of four domains: ZU5, SH3, and two C-terminal death domains (DD). Expression of MACC1 was found significantly upregulated in malignant tissues (colon cancer of all stages as well as liver and lung metastases) compared to normal tissues or adenomas. MACC1 represents an early and crucial prognostic indicator for colon cancer metastasis that is independent of age, sex, tumor infiltration, nodal status, and lymph vessel invasion. Besides its involvement in signal transduction with the MET receptor, MACC1 also links MET signaling and apoptosis. MACC1 may also be an important therapeutic target for colorectal cancer treatment. At least two isoforms of MACC1 are known to exist. Anti-MACC1 antibody is ideal for investigators involved in cell cycle protein and cancer research.
Immunogen
MACC1 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide corresponding to an internal region of human MACC1.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-MACC1 antibody has been tested in ELISA, WB, and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~91-96 kDa in size corresponding to MACC1 by Western blotting in the appropriate cell lysate or extract. Mouse liver tissue lysate may be used as positive control.
Purity/Specifity
Anti-MACC1 Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with MACC1 with Human, Rat and Mouse based on 100% homology with the immunizing sequence. Cross-reactivity with MACC1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Arlt F and Stein U. (2009) Colon cancer metastasis: MACC1 and Met as metastatic pacemakers. Int. J. Biochem. Cell Biol. epub. Stein U, Walther W, Arlt F, et al. (2009) MACC1, a newly identified key regulator of HGF-MET signaling, predicts colon cancer metastasis. Nat. Med. 15:59-67. Boardman LA. (2009) Overexpression of MACC1 leads to downstream activation of HGF/MET and potentiates metastasis and recurrence of colorectal cancer. Genome Med. 1:36. Stein U, Smith J, Walther W, et al. (2009) MACC1 controls Met: what a difference an Sp1 site makes. Cell Cycle 2009; 8:2467-9.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
600-401-989

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
600-401-989S

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry