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Primary Antibodies  >  Sample Size Primary Antibodies
 

ABCB5 Antibody

Rabbit Polyclonal
Code

600-401-A77S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
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Immunohistochemistry of ABCB5 antibody Tissue: human liver Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: ABCB5 1:200 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: Moderate to strong cytoplasmic and membranous staining was observed in hepatocytes. Occasional nuclear staining was observed in hepatocytes and sinusoidal cells. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain
Western blot using Rockland's affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10,000 dilution in 5% milk in TBST at 4° C, overnight. Personal Communication, JP Gillet, CCR-NCI, Bethesda, MD.
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Synonyms: ATP binding cassette sub family B (MDR/TAP) member 5 antibody, ATP binding cassette sub family B member 5 antibody, EST422562 antibody, P glycoprotein ABCB5 antibody

ABCB5 Antibody Properties

Description
Anti-ABCB5 (RABBIT) Antibody - 600-401-A77S
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:15,000
Western Blot : 1:10,000
Immunochemistry: 1:200
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.31 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
ABCB5
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Lot#  

ABCB5 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-ABCB5 Antibody is a ATP-Binding Cassette protein. ABCB5 is a novel member of the human P-glycoprotein family.  It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells.  Cell fusion is thought to contribute to tissue differentiation.  ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes.  ABCB5 is a rhodamine efflux transporter.  This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5. ABCB5 antibody is ideal for Cancer and Signal Transduction research.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This affinity purified antibody has been tested for use in ELISA, IHC, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 117 kDa in size corresponding to ABCB5 by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human ABCB5 protein.  A BLAST analysis was used to suggest partial cross-reactivity with ABCB5 from monkey (85% homology), rat (68% homology) and mouse (62% homology) sources.  Cross-reactivity with ABCB5 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH. (2005)  ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 65(10):4320-33. Chen KG, Szakacs G, Annereau JP, Rouzaud F, Liang XJ, Valencia JC, Nagineni CN, Hooks JJ, Hearing VJ, Gottesman MM. (2005) Principal expression of two mRNA isoforms (ABCB 5alpha and ABCB 5beta ) of the ATP-binding cassette transporter gene ABCB 5 in melanoma cells and melanocytes.  Pigment Cell Res. 18(2):102-12. Chen KG, Gottesman MM.  (2005) Useful tool to generate unidirectional deletion vectors by utilizing the star activity of BamHI in an NcoI-BamHI-XhoI cassette.  Biotechniques. 38(2):198, 200, 202, 204.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-B99

Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-C23

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Bovine
Catalog Number
200-401-B74

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Bovine
Catalog Number
200-401-B74S

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry