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Primary Antibodies  >  Stem Cell Antibodies
 

Sipa1 Antibody

Rabbit Polyclonal
Code

600-401-A36
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Rockland's affinity purified anti-Sipa1 antibody was used at 1.25 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate to strong positive staining of lymphocytes within human tonsil at 40X.  Tissue was formalin-fixed and paraffin embedded.  The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.  Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Western blot using Rockland's affinity purified anti-Sipa1 antibody shows detection of over-expressed Sipa1 in lysates from mouse 3T3 cells transfected with Sipa1 (lane 1).  Endogenous Sipa1 is detected in lane 2, which contains lysate from 3T3 cells mock-transfected with LacZGLB, although at a significantly reduced level compared to transfected cells.  Lane 3 and 4 are similar to lanes 1 and 2 except the antibody was preincubated with the immunizing peptide prior to reaction with the membrane.  The identity of the higher and lower molecular weight bands is unknown.  The band at ~130 kDa, indicated by the arrowhead, corresponds to recombinant Sipa1.  Primary antibody was used at 1:1250. Personal communication, H. Yang, L. Lukes and K. Hunter, NCI, Bethesda, MD.
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Synonyms: GTPase activating protein Spa 1 antibody, MGC102688 antibody, MGC17037 antibody, p130 SPA1 antibody, Signal induced proliferation associated 1 antibody

Sipa1 Antibody Properties

Description
Anti-Sipa1 (RABBIT) Antibody - 600-401-A36
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse, human, rat
Clonality
Polyclonal
Application
ELISA : 1:20,000
Western Blot : 1:1,000 - 1:5,000
Immunochemistry: 1.25-5 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.25 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SIPA1
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Lot#  

Sipa1 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Sipa1 (signal-induced proliferation associated gene 1) is a mitogen-induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. Sipa1 is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of mouse Sipa1.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 130 kDa in size corresponding
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  This antibody is specific for mouse Sipa1 protein.  A BLAST analysis was used to suggest cross-reactivity with Sipa1 from mouse, human and rat based on a 100% homology with the immunizing sequence.  Cross-reactivity with Sipa1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Kits;
Applications
ELISA, WB, IHC, IF, IP
Catalog Number
K-500

Product Type
Supporting Reagents;
Applications
ELISA, WB, IHC, IF
Catalog Number
K505

Product Type
Molecular Biology Reagents;
Catalog Number
MB-015

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry