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Primary Antibodies  >  Cancer Research Antibodies
 

MECT1 Antibody

Rabbit Polyclonal
Code

600-401-936
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western blot using Rockland's affinity purified anti-MECT2 antibody shows detection of MECT2 (lower arrowhead) and MECT2:MAML2 fusion protein (top arrowhead) in cell lysates.  Lane 1 contains lysate from cells expressing MECT2 only.  Lane 2 contains lysate from cells transfected with fusion protein.  Lane 3 contains lysate from cells strongly expressing MECT2 and weakly expressing the fusion protein.  Lane 4 contains lysate from control cells.  After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Frederick Kaye, CCR-NCI, Bethesda, MD.
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Synonyms: CREB regulated transcription coactivator 1 antibody, CRTC1 antibody, KIAA0616 antibody, MECT 1 antibody, Mucoepidermoid carcinoma translocated protein 1 antibody

MECT1 Antibody Properties

Description
Anti-MECT1(RABBIT) Antibody - 600-401-936
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat, zebrafish, pufferfish, bovine
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:40,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.95 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CRTC1
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Lot#  

MECT1 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. MECT1 (also known as MucoEpidermoid Carcinoma Translocated 1, Transducer of regulated cAMP response element-binding protein 1, TORC1, and Transducer of CREB protein 1) is a nuclear protein that functions as a transcriptional coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites. MECT1does not appear to modulate CREB1 DNA-binding activity but
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 19-34 of human MECT1 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80-95 kDa in size corresponding to MECT1 protein by western blotting in the appropriate cell lysate or extract.  This antibody is will react with isoform 1 and 2 of MECT1.
Purity/Specifity
This affinity-purified antibody is directed against human MECT1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross reactivity with MECT1 protein from human, mouse, rat, zebrafish, pufferfish and bovine based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, GS, Other
Reacts With
human, mouse, rat
Catalog Number
100-401-195

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
600-401-270

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry