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Primary Antibodies > Cancer Research Antibodies

Mesothelin Antibody

Mouse Monoclonal MB-G10 IgG2a

Code

200-301-A87

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Immunohistochemistry using Rockland's anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission fromClin.Cancer Res. 11(16):5840-6.

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Synonyms: Mesothelian, MN, MB, Pre-pro-megakaryocyte-potentiating factor, CAK1 antigen

Datasheet
References
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Mesothelin Antibody Properties

Description

Anti-Mesothelin (MOUSE) Monoclonal Antibody - 200-301-A87

Host

Mouse

Target Species

Human

Known Cross Reactivity

human

Clonality

Monoclonal MB-G10 IgG2a

Application

ELISA : 1:10,000 - 1:50,000
Western Blot : 1:1,000
Immunohistochemistry: 1:100
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

MSLN

Mesothelin Antibody Description

Background

Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.

Immunogen

This antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to the extracellular domain of human mesothelin.

Immunogen Type

Recombinant Protein

Storage Condition

Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For Anti-mesothelin immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.

Purity/Specifity

This antibody is directed against human mesothelin protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. Cross reactivity with homologues from other sources has not been tested.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Chang K, Pastan I, Willingham MC. (1992) Isolation and characterization of a monoclonal antibody reactive with ovarian cancers and normal mesothelium. Int J Cancer 50:373–81. Argani P, Iacobuzio-Danahue C, Ryu B, et al. (2001) Mesothelin is overexpressed in the vast majority of ductal adenocarcinomas of the pancreas: identification of a new pancreatic cancer marker by serial analysis of gene expression (SAGE). Clin Cancer Res 7:3862–8. Chang K, Pastan I.(1996) Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesothelioma, and ovarian cancers. Proc Natl Acad Sci U S A 93:136–40.

Specific Reference

Onda, M., Willingham, M., Nagata, S., Bera, T.K., Beers, R., Ho, M., Hassan, R., Kreitman, R.J., Pastan, I. (2005) New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA. Clin Cancer Res. 11(16):5840-6. Ho, M., Bera, T.K., Willingham, M., Onda, M., Hassan, R., Fitzgerald, D. and Pastan, I. (2007) Mesothelin Expression in Human Lung Cancer. Clin Cancer Res 13:1571-5.

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra

ELISA