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Primary Antibodies > Stem Cell Antibodies

Wnt1 Antibody

Mouse Monoclonal 13F9 IgG1 kappa

Code

200-301-A37

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's Protein A purified anti-Wnt1 monoclonal antibody shows detection of Wnt1 protein in mouse testis lysate. The results show specific binding corresponding to the ~41 kDa Wnt1 protein. Primary antibody was used at a 1:500 dilution. Personal communication, Stephen Brown, Brown University

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Synonyms: INT1, Proto-oncogene protein Wnt-1

Datasheet
References
Customer Reviews
Protocols
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Wnt1 Antibody Properties

Description

Anti-Wnt1 (MOUSE) Monoclonal Antibody - 200-301-A37

Host

Mouse

Target Species

Human

Known Cross Reactivity

human

Clonality

Monoclonal 13F9 IgG1 kappa

Application

ELISA : 1:4,000 - 1:20,000
Western Blot : 1:175 - 1:250
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

WNT1

Wnt1 Antibody Description

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98% identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.

Immunogen

Synthetic peptide corresponding to an internal region of human Wnt1 protein.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to Wnt1 by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with human and mouse Wnt1 protein. A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, opossum and rat based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology. Cross-reactivity with Wnt1 from other sources has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

De Ferrari,G.V. and Moon,R.T. (2006) The ups and downs of Wnt signaling in prevalent neurological disorders. Oncogene 25 (57), 7545-7553. Yook,J.I., Li,X.Y., Ota,I., Hu,C., Kim,H.S., Kim,N.H., Cha,S.Y., Ryu,J.K., Choi,Y.J., Kim,J., Fearon,E.R. and Weiss,S.J. (2006) A Wnt-Axin2-GSK3beta cascade regulates Snail1 activity in breast cancer cells. Nat. Cell Biol. 8 (12), 1398-1406. He,J., Sheng,T., Stelter,A.A., Li,C., Zhang,X., Sinha,M., Luxon,B.A. and Xie,J. (2006) Suppressing Wnt signaling by the hedgehog pathway through sFRP-1. J. Biol. Chem. 281 (47), 35598-35602.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

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DyLight™ Antibody Spectra