Immunofluorescence results using Avidin CY3.5

Datasheet

Avidin Cy3.5 Conjugated

Name: Avidin Cy3.5 Conjugated
Brand: Rockland Immunochemicals
Price: 189.0 USD
Availability: InStock

2 References

Item No. A003-12

Size: 1 mg

Applications: IF, FISH, Multiplex

$189.00 /Per Item

Add To Cart

Add to List

Bulk Orders

Availability: Ships next business day

Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Avidin Cy3.5 Conjugated is suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Expand Background

Specifications for Avidin Cy3.5 Conjugated

Product Details

Description: Avidin CY3.5 Conjugated - A003-12

Synonyms: Avidin Cy3.5 Conjugated

Conjugate: Cy3.5™

Target Details

Purity/Specificity: Avidin Cy3.5 Conjugated was prepared from chromatographically purified Avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin.

Application Details

Suggested Applications: FISH, IF, Multiplex - View References

Application Note: Avidin Cy3.5 Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting.

Formulation

Physical State: Lyophilized

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (2)

Pfleghaar KB et al. (2015). Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells. Nucleus.

Applications

IF, Confocal Microscopy

PubMed

Kraus J et al. (2003). Multicolour FISH fine mapping unravels an insertion as a complex chromosomal rearrangement involving six breakpoints and a 5.89 Mb large deletion. J Mes Genet.

Applications

ISH, FISH; Multiplex Assay

PubMed

Related Protocols

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.