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Primary Antibodies > Apoptosis Antibodies

FKBP8 Antibody

Rabbit Polyclonal

Code

200-401-A58

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 µg of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4° C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.

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Synonyms: 38 kDa FK 506 binding protein homolog antibody, 38kDa antibody, FK506 binding protein 8 antibody, FKBP 38 antibody

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FKBP8 Antibody Properties

Description

Anti-FKBP8 (RABBIT) Antibody - 200-401-A58

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human, mouse, rat

Clonality

Polyclonal

Application

ELISA : 1:25,000
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: User Optimized
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

2.3 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

FKBP8

FKBP8 Antibody Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. FKBP8 (also known as FK506-binding protein 38) is a member of the immunophilin family that has been implicated to play an important role in apoptosis through its involvement in the mechanism that targets Bcl-2 and Bcl-xL to the outer mitochondrial membrane (OMM). Suppression of endogenous FKBP8 by RNAi or transfection of a mutant FKBP8 missing the transmembrane domain necessary for mitochondrial insertion, resulted in the translocation of Bcl-2 and Bcl-xL from the OMM to the cytosol. It has also been suggested that FKBP8 may play

Immunogen

This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human FKBP8 protein.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 64 kDa in size corresponding to FKBP8 by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human FKBP8 protein. A BLAST analysis was used to suggest partial cross-reactivity with FKBP8 from mouse and rat sources based on ~93% homology with the immunizing sequence. Cross-reactivity with FKBP8 from other sources has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Portier,B.P. and Taglialatela,G. (2006) Bcl-2 localized at the nuclear compartment induces apoptosis after transient overexpression. J. Biol. Chem. 281 (52), 40493-40502. Okamoto,T., Nishimura,Y., Ichimura,T., Suzuki,K., Miyamura,T., Suzuki,T., Moriishi,K. and Matsuura,Y. (2006) Hepatitis C virus RNA replication is regulated by FKBP8 and Hsp90. EMBO J. 25 (20), 5015-5025. Wang,J., Tong,W., Zhang,X., Chen,L., Yi,Z., Pan,T., Hu,Y., Xiang,L. and Yuan,Z. (2006) Hepatitis C virus non-structural protein NS5A interacts with FKBP38 and inhibits apoptosis in Huh7 hepatoma cells. FEBS Lett. 580 (18), 4392-4400. Maestre-Martinez,M., Edlich,F., Jarczowski,F., Weiwad,M., Fischer,G. and Lucke,C. (2006) Solution structure of the FK506-binding domain of human FKBP38. J. Biomol. NMR 34 (3), 197-202. Rosner,M., Hofer,K., Kubista,M. and Hengstschlager,M. (2003) Cell size regulation by the human TSC tumor suppressor proteins depends on PI3K and FKBP38. Oncogene 22 (31), 4786-4798. Shirane,M. and Nakayama,K.I. (2003) Inherent calcineurin inhibitor FKBP38 targets Bcl-2 to mitochondria and inhibits apoptosis. Nat. Cell Biol. 5 (1), 28-37.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

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