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Primary Antibodies  >  Apoptosis Antibodies
 

RFFL Antibody

Rabbit Polyclonal
Code

200-401-932
Size

500 µg
Price

$309.00
Availability

Ships next business day.
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Rockland's Affinity Purified anti-RFFL antibody shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue.  Tissue was formalin-fixed and paraffin embedded.  Brown color indicates presence of protein, blue color shows cell nuclei.   Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
Western blot using Rockland's Protein A Purified anti-RFFL antibody shows detection of RFFL (arrowhead) in lysate.  Lanes correspond to empty vector 293T cell lysate (mock, left); RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 µl of lysate per lane.  Lysates were prepared from equivalent numbers of cells.  Data presented demonstrate that this reagent is specific for RFFL.  After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000 using 5% BLOTTO, 0.1% Tween-20 in PBS as the diluent.  Incuba-tion occurred for 1 h at room temperature. Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.
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Synonyms: RFFL, Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like

RFFL Antibody Properties

Description
Anti-RFFL (RABBIT) Antibody - 200-401-932
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:4,000 - 1:20,000
Western Blot : 1:500 - 1:3,000
Immunohistochemistry: 1:500 - 1:3,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
5.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
500 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
RFFL
Download CofA
Lot#  

RFFL Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. RFFL, also known as Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like, is a novel modulator of NF-kB activation.  RFFL possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RFFL also possesses anti-apoptotic activity and may bind phosphatidylinositol phosphates. RFFL is a membrane bound cytoplasmic protein that is expressed ubiquitously. RFFL can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes and is rapidly degraded after stimulation with TNFSF10, and probably by caspases. Multiple transcript variants have been detected for this protein.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-363 of human RFFL protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RFFL protein by western blotting in the appropriate tissue or cell lysate or extract.  Isoforms 2 and 3 are 39.7 and 36.6 kDa, respectively and should also cross react with this antibody.
Purity/Specifity
This protein A purified antibody is directed against human RFFL protein. The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.  A BLAST
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Coumailleau F, Das V, Alcover A, Raposo G, Vandormael-Pournin S, Le Bras S, Baldacci P, Dautry-Varsat A, Babinet C, Cohen-Tannoudji M. (2004) Over-expression of Rififylin, a new RING finger and FYVE-like domain-containing protein, inhibits recycling from the endocytic recycling compartment. Mol Biol Cell 15(10):4444-56. McDonald,E.R. III and El-Deiry,W.S. (2004) Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth. Proc. Natl. Acad. Sci. U.S.A. 101 (16), 6170-6175. Tibbetts,M.D., Shiozaki,E.N., Gu,L., McDonald,E.R. III, El-Deiry,W.S. and Shi,Y. (2004) Crystal structure of a FYVE-type zinc finger domain from the caspase regulator CARP2. Structure (Camb.) 12 (12), 2257-2263.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
200-401-A34

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
Human, Mouse, Rat
Catalog Number
600-401-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat, Xenopus
Catalog Number
600-401-964

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, mouse, rat
Catalog Number
600-401-966

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry