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GFP and Epitopes  >  Antibodies to GFP and Antibodies to RFP
 

GFP Antibody Biotin Conjugated

Rabbit Polyclonal
Code

600-406-215
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Immuno-Fluorescence of Biotin Mouse anti-GFP antibody.  
Biotin mouse anti GFP used 1:5000 As referenced in:
Lu S-M, Tremblay M-E` , King IL, Qi J, Reynolds HM, et al. (2011) HIV-1 Tat-Induced Microgliosis and Synaptic Damage via Interactions between Peripheral  and Central Myeloid Cells. PLoS ONE 6(9): e23915. doi:10.1371/journal.pone.0023915
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$40.00 to United States
Handling:
$20.00
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.

GFP Antibody Biotin Conjugated Properties

Description
Anti-GFP (RABBIT) Antibody Biotin Conjugated - 600-406-215
Host
Rabbit
Known Cross Reactivity
wt, rGFP, eGFP
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
IF Microscopy : 1:5,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Epitope Tag Type
GFP
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

GFP Antibody Biotin Conjugated Description

Background
GFP Antibody is produced from Green fluorescent protein which is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Immunogen
Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type
Recombinant Protein
Storage Condition
Store Anti-GFP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Biotin Conjugated GFP Antibody is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Purity/Specifity
Anti-GFP was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria).  No reaction was observed against Human, Mouse and Rat Serum Proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Zangar RC, Daly DS, White AM, et al. ProMAT calibrator: A tool for reducing experimental bias in antibody microarrays. J. Proteome Res. 2009 Aug;8(8):3937–3943. Lu S-M, Tremblay M-È, King IL, et al. HIV-1 Tat-induced microgliosis and synaptic damage via interactions between peripheral and central myeloid cells. PLoS ONE. 2011;6(9):e23915.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Biotin
Color
n/a
Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
455
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester