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GFP and Epitopes  >  Antibodies to GFP and Antibodies to RFP
 

GFP Monoclonal Antibody

Mouse Monoclonal 9F9.F9 IgG1 kappa
Code

600-301-215
Size

1 mg
Price

$365.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
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Mouse monoclonal Anti-GFP antibody is able to detect positive cell population in cytoflow analysis.  A. cells were not stained with anti-GFP antibody/fluorescence complex. B. Cells were stained with anti-GFP antibody/fluorescence complex and sorted. All recovered individual cells sorted by FACS have been re-confirmed to be positive.
Western Blot of Mouse anti-GFP antibody.  
Lane 1:  Molecular Weight Markers.  
Lane 2:  GFP.  
Load:  10ng per lane.
Primary antibody:  Ms Anti-GFP antibody at 1:1000 for overnight at 4°C.
Secondary antibody:  Mouse HRP secondary antibody at 1:40,000 for 30 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  27 kDa, 27 kDa for epitope tag GFP. 
Other band(s): None.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.

GFP Monoclonal Antibody Properties

Description
Anti-GFP (MOUSE) Monoclonal Antibody - 600-301-215
Host
Mouse
Known Cross Reactivity
wt, rGFP, eGFP
Clonality
Monoclonal 9F9.F9 IgG1 kappa
Application
ELISA : 1:80,000 - 1:500,000
IF Microscopy : User Optimized
FlowCytometry : User Optimized
Western Blot : 1:3,000 - 1:30,000
Immunochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Epitope Tag Type
GFP
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG1
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

GFP Monoclonal Antibody Description

Background
Mouse anti-GFP antibody is functional by western blot, ELISA, Immunofluorescene Micorscopy and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Immunogen
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
Immunogen Type
Recombinant Protein
Storage Condition
Store mouse anti-GFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Purity/Specifity
GFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215) from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Yang F, Camp DG 2nd, Gritsenko MA, et al. Identification of a novel mitotic phosphorylation motif associated with protein localization to the mitotic apparatus. J. Cell. Sci. 2007 Nov 15;120(Pt 22):4060–4070. Shen W, Da Silva JS, He H, Cline HT. Type A GABA-receptor-dependent synaptic transmission sculpts dendritic arbor structure in Xenopus tadpoles in vivo. J. Neurosci. 2009 Apr 15;29(15):5032–5043. Koenig A, Mueller C, Hasel C, et al. Collagen type I induces disruption of E-cadherin-mediated cell-cell contacts and promotes proliferation of pancreatic carcinoma cells. Cancer Res. 2006 May 1;66(9):4662–4671. Jesse S, Koenig A, Ellenrieder V, Menke A. Lef-1 isoforms regulate different target genes and reduce cellular adhesion. Int. J. Cancer. 2010 Mar 1;126(5):1109–1120. Goel M, Sienkiewicz AE, Picciani R, et al. Cochlin induced TREK-1 co-expression and annexin A2 secretion: role in trabecular meshwork cell elongation and motility. PLoS ONE. 2011;6(8):e23070. Forrest JC, Paden CR, Allen RD, et al. ORF73-Null Murine Gammaherpesvirus 68 Reveals Roles for mLANA and p53 in Virus Replication. J Virol. 2007 Nov;81(21):11957–11971.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry