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Background

Anti-Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti-FITC is also suitable as a visualization reagent for fluorescein-labelled compounds (such as antibodies, lectins and peptide nucleic acid (PNA) probes).

Product Details

Anti-Fluorescein (GOAT) Antibody - 600-101-096
goat Anti FITC, goat anti Fluorescein
Goat
Polyclonal
IgG

Target Details

Fluorescein
Other
Fluorescein conjugated to Goat IgG
Fluorescein Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein conjugated Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.

Application Details

ELISA, WB
FISH, Microarray  - View References
Anti-Fluorescein Antibody has been tested by ELISA and western blot and is suitable for immunomicroscopy, in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Formulation

Liquid (sterile filtered)
1.2mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). Disposable photonics for cost-effective clinical bioassays: application to COVID-19 antibody testing. Lab Chip.
Applications
Microfluidic Point of Care Chip Immunoassay
(). Robustness of the Dorsal morphogen gradient with respect to morphogen dosage. PLoS Comput Biol.
Applications
ISH, FISH
(). Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems. PLoS One
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ISH, FISH
(). Bach1 siRNA attenuates bleomycin-induced pulmonary fibrosis by modulating oxidative stress in mice. International Journal of Molecular Medicine
Applications
WB, IB, PCA
(). Activation of cannabinoid receptor type 2 by JWH133 alleviates bleomycin-induced pulmonary fibrosis in mice. Oncotarget
Applications
WB, IB, PCA
(). Survival of irradiated recipient mice after transplantation of bone marrow from young, old and "early aging" mice. Aging
Applications
ISH, FISH
(). A Multiplex Label-Free Approach to Avian Influenza Surveillance and Serology. PloS One
Applications
Microarray, Microarray chip
(). Direct and indirect contribution of bone marrow‐derived cells to cancer. Int J Cancer.
Applications
ISH, FISH

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.