14-12_peptide top banner
 
GFP and Epitopes  >  Antibodies to Fluorescent Tags
 

Fluorescein (FITC) Antibody

Goat Polyclonal
NCI Collaboration
Order Offline
Western Blot of Goat Anti-Fluorescein antibody.
Lane 1:  BSA conjugated Fluoresccein.
Lane 2:  None.
Load:  50 ng per lane.
Primary antibody:  Fluorescein primary antibody at 1:1,000 overnight at 4°C.
Secondary antibody:  Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT.
Blocking:  MB-070 for 30 min at RT.
Predicted/Observed size:  67 kDa, 67 kDa for Fluorescein.
Other band(s):  None.
View All Images
Number:
600-101-096
Size:
1 mg
Price:
$215.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Fluorescein (FITC) Antibody Properties

Description
Anti-Fluorescein (GOAT) Antibody - 600-101-096
Host
Goat
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 - 1:3,000
Immunohistochemistry: 1:250 - 1:1,500
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
IgG
Label
Concentration
1.0mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
Download CofA:
Lot#  
Print

Fluorescein (FITC) Antibody Description

Background
Anti-Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti-FITC is also suitable as a visualization reagent for fluorescein-labelled compounds (such as antibodies, lectins and peptide nucleic acid (PNA) probes).
Synonyms
Anti-FITC
Immunogen
Fluorescein conjugated to Goat IgG
Immunogen Type
Other
Storage Condition
Store vial at 4° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
Anti-Fluorescein Antibody is suitable for immunomicroscopy, in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Purity/Specifity
Fluorescein Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein conjugated Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Noga E. J., Udomkusonsri, P. (2002). Fluorescein: A Rapid, Sensitive, Nonlethal Method for Detecting Skin Ulceration in Fish. Vet Pathol 39 (6): 726–731(6). doi:10.1354/vp.39-6-726. PMID 12450204.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry