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GFP and Epitopes  >  Antibodies to FLAG™ and Antibodies to 6XHIS Tags

6X HIS tagged Antibody DyLight™ 649

Mouse Monoclonal 33D10.D2 IgG1 kappa


100 µg


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ELISA of Mouse anti-6xHIS Tag Antibody.
Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins.
Coating amount: 0.15ug per welll.
Primary antibody: 6xHIS Tag antibody at 100ug/mL.
Dilution series: 2-fold.
Mid-point concentration: 200ng/mL.
Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000.
Substrate: TMB (p/n TMBE-1000).
Western Blot of Mouse Anti-6X HIS EPITOPE TAG Monoclonal Antibody DyLight™ 649 Conjugated
Lane 2:  Recombinant 6xHIS-SUMO-GFP   
Lane3:  Nag1
Load:  Lane 2 - 100 ng
Primary antibody: 6X HIS EPITOPE TAG Monoclonal Antibody DyLight™ 649 Conjugated at 1:1000 30 min RT
Block:  1% BSA-TTBS 30 min RT
Observed size:  Lane  2 - 45 kDa, Lane 3 - 16 and 32 kDa
Other band(s):  Lysate breakdown
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$40.00 to United States
Synonyms: anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

6X HIS tagged Antibody DyLight™ 649 Properties

Anti-6X HIS EPITOPE TAG (MOUSE) Monoclonal Antibody DyLight™ 649 Conjugated - 200-343-382
Monoclonal 33D10.D2 IgG1 kappa
FLISA : >1:20,000
IF Microscopy : >1:5,000
Western Blot : >1:10,000-1:25,000
Epitope Tag Type
Physical State
Shipping condition
FP Value
1.9 moles DyLight™ 649 per mole of IgG1
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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6X HIS tagged Antibody DyLight™ 649 Description

Anti 6X HIS tagged Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. Anti-6X HIS Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Immunogen Type
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.  To date, this antibody has reacted with all His tagged proteins so far tested.  In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.  The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Brandsma, M ; X Wang, H Diao, SE. Kohalmi1, AM Jevnikar and S. Ma (2009) A Proficient Approach to the Production of Therapeutic Glucagon-Like Peptide-1 (GLP-1) in Transgenic PlantsThe Open Biotechnology Journal, 3, 57-66 57 1874-0707/09
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Product Label


DyLight™ 649
Far Red
Conjugation Reference
Modified from Thermo Fisher Scientific Inc, Rockford, IL
Molecular Weight
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester