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Primary Antibodies  >  Phospho Specific Antibodies
 

AKT pS473 Antibody

Rabbit Polyclonal
Code

600-401-268
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Immunohistochemistry of Rabbit Anti-Akt pS473 antibody.  
Tissue:  human breast carcinoma.  
Fixation:  formalin fixed paraffin embedded.  
Antigen retrieval:  not required.
Primary antibody:  Akt pS473 antibody  at 100 dilution for 1 h at RT.
Secondary antibody:  Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT.
Localization:  Akt pS473 is nuclear and occasionally cytoplasmic.
Western Blot of Rabbit anti-AKT pS473 antibody.  
Lane 1:  nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase.    
Load:  35 µg per lane.
Primary antibody:  AKT pS473 antibody at 1:200 dilution for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  ~56 kDa for AKT pS473. 
Other band(s): unspecific.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

AKT pS473 Antibody Properties

Description
Anti-AKT pS473 (RABBIT) Antibody - 600-401-268
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Application
ELISA : 1:15,000 - 1:60,000
FlowCytometry : User Optimized
Western Blot : 1:200 - 1:1000
Immunochemistry: 1:100 - 1:500
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.02 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
AKT1
Download CofA
Lot#  

AKT pS473 Antibody Description

Background
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at  Ser 473 and Thr 308.  AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways.  AKT Exhibits tight control over cell proliferation and cell viability.  Overexpression or inappropriate activation of AKT is noted in many types of cancer.   AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phos-phorylated, AKT dissociates from the membrane and phos-phorylates targets in the cytoplasm and the cell nucleus.  AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminus aa 460-480 of human, mouse, rat and chicken AKT proteins conjugated to KLH.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antibody is phospho specific for pS473 and is suitable for western blotting, immunohistochemistry (formalin-fixed paraffin-embedded sections), immunofluorescence and ELISA. By immunoblot a single band of the expected apparent molecular weight is observed.  For immunohistochemistry no pre-treatment of sample is required.
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.  This antibody is specific for phosphorylated human AKTpS473.  Minimal reactivity occurs against non-phosphorylated AKT.  Reactivity against AKT from other species may occur but has not yet been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses.  J. Cell Science 114:2903-2910. Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14.
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
000-000-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-269

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, chimpanzee
Catalog Number
200-301-401

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry