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Standard HCP Reagent Development


Standard HCP Reagent Development

 

The Standard  method is a process is  where the two sets of animals are immunized using high and low molecular weight Host Cell Protein (HCP) extract.  After immunization and primary boosting, antisera is collected.  The developed anti-HCP antibodies are evaluated to determine coverage of potential HMW  and LMW host cell contaminants  . The final HCP antibody product is a pool of anti-HMW and anti-LMW antibodies having broad coverage of the HCP antigens in the final antibody reagent. Coverage is demonstrated by 1D western blot analysis  or 2D western Blot Analysis against the HCP extract.  HCP-detection assay development is available.  To obtain the optimal level of coverage multiple host animals may be utilized. The following species are available; Rabbit, Chicken, Sheep, Goat and Donkey.

 

 

Standard Host Cell Protein

 

 

 

Related Links:

 

Host Cell Protein


HCP Assay Development


Cascade Antibody Generation


1D Analysis


2D Analysis